MicroRNA-151a-3p Increases In Human Pancreatic Cancer Cells Colonization,Apoptosis And Cycle Effects

Objective: To detect the expression of miR-151a-3p in different human pancreatic cancer cells,and to study the effect of miR-151a-3p on proliferation,apoptosis and cell cycle of human pancreatic cancer cells.Methods: The clinical data of pancreatic cancer associated with miR-151 a were screened from the TCGA database.Statistical analysis was performed using GraphPad Prism 5.02 and SPSS 22.0 Statistics software to obtain the expression level of miR-151 a in pancreatic cancer tissues and normal tissues,as well as the association with the five-year survival of patients with pancreatic cancer,and tumor resected max dimension.The expression levels of miR-151a-3p in SW1990,PANC-1,AsPC-1 and BxPC-3 human pancreatic cancer cells were detected by qRT-PCR.Two relatively high expression strains were selected and miR-151a-3p mimic,miR-151a-3p inhibitor were transferred to them by RNA interference technology.Then,the effect of miR-151a-3p on the proliferation,apoptosis and cycle of human pancreatic cancer cells was detected by EdU cell proliferation assay kit and flow cytometry,which can show the effect of miR-151a-3p on the function of human pancreatic cancer cells.Results:1.miR-151 a showed differential expression in pancreatic cancer patients,and there was no significant difference compared with normal pancreatic tissue(P>0.05).2.Statistical analysis of 5-year survival of patients with pancreatic cancer showed that patients with high expression of miR-151 a had a poor prognosis(P=0.0076).3.There was no significant correlation between the expression level of miR-151 a and tumor resected max dimension of patients with pancreatic cancer,and the difference was not statistically significant(P=0.9789).4.qRT-PCR results showed that miR-151a-3p was differentially expressed in four human pancreatic cancer cells(PANC-1>BxPC-3>AsPC-1>SW 1990).5.The miR-151a-3p mimic and miR-151a-3p inhibitors were transfected into PANC-1 and BxPC-3 pancreatic cancer cell lines by RNA interference technology.The results of qRT-PCR showed that the expression of miR-151a-3p was increased in PANC-1 and BxPC-3 cells after miR-151a-3p mimic intervention;the results of immunofluorescence tracer showed that fluorescent expression was observed in PANC-1 and BxPC-3 cells fafter miR-151a-3p inhibittor intervention.6.Cell proliferation results showed that the proliferation of BxPC-3 pancreatic cancer cell lines treated with miR-151-3p mimic was enhanced,while the proliferation of BxPC-3 pancreatic cancer cell lines treated with miR-151-3p inhibitor was weakened(P< 0.05),there was no significant change in the proliferation ability of PANC-1 pancreatic cancer cell lines(P>0.05).7.Apoptosis results showed that the apoptosis rate of PANC-1 cell line was increased after down-regulated miR-151a-3p expression(P<0.05),and the apoptosis of BxPC-3 cell line was not changed(P>0.05).There was no significant change in the apoptosis of PANC-1 cell line after up-regulation of miR-151a-3p expression(P>0.05),and the apoptosis rate of BxPC-3 cell line was decreased(P<0.05).8.Cell cycle results showed that compared with the control group,the PANC-1 cell line with high expression of miR-151a-3p showed a shortened G1-phase,and the PANC-1 cell line with low expression of miR-151a-3p showed a G1-phase delay,the difference was statistically significant(P<0.05);compared with the control group,the cell cycle of BxPC-3 cell line with high-expression and low-expression of miR-151a-3p did not change significantly.There was no significant difference(P>0.05).Conclusion:1.miR-151 a is differentially expressed in pancreatic cancer.The lower the expression level,the better the survival prognosis of patients with pancreatic cancer.2.miR-151a-3p can promote the cell proliferation,inhibit cell apoptos-is in pancreatic cancer cells and participate in the regulation of G1-phase.