Screening And Function Research Of Key MiRNA From Patients With Laryngeal Carcinoma And Hypopharyngeal Carcinoma

Background and objective:Head and neck squamous cell carcinoma(HNSCC)is a common and highly invasive malignancy originated from the skin and mucosal epithelial cells,including nasopharyngeal carcinoma,oropharyngeal cancer,hypopharyngeal carcinoma and laryngeal cancer.The main pathological type of head and neck malignant tumors is HNSCC,which is characterized with potent local invasion ability and easy metastasis to surrounding and distant lymph nodes,with constantly low five-year overall survival rate.According to statistics,there are 500,000 new cases of HNSCC annually worldwide.At present,smoking,drinking and human papillomavirus(HPV)infection are considered to be associated with the occurrence of HNSCC.The treatment of early stage HNSCC is mainly based on surgery or radiochemotherapy,while that of advanced HNSCC is mainly based on multidisciplinary treatment.Laryngeal cancer and hypopharyngeal carcinoma are the most common in all types of HNSCC.In spite of the diverse therapeutic approaches at present,the survival rate of patients is still relatively low.The massive reports on the relationship between the abnormal expression of microRNAs(miRNAs)in HNSCC and the pathogenesis and progression of HNSCC have provided great help for us to understand the molecular mechanism of the pathogenesis and progression of laryngeal and hypopharyngeal carcinoma.miRNAs are small,non-coding RNAs with approximately 21-25 nucleotides in length.These non-coding genes regulate the expression of target genes at the post-transcriptional level or inhibit the translation of target proteins.The specific mechanism is that miRNAs bind to the 3'UTR on mRNA of target genes,which regulates the signaling pathway involved in its target gene,thereby playing roles as oncogenes and tumor suppressor genes.Therefore,the elucidation of the functional role of miRNAs in human precancerous lesions would further enrich the prevention and therapeutic approaches of tumors,which is of great significance for promoting anti-cancer.The abnormal expression of miRNAs in human tissues and blood is closely associated with the pathogenesis,progression and therapeutic response of cancer,which can be very promising research objects of tumor marker recognition.Therefore,the exploration of specific miRNAs between laryngeal,hypopharyngeal,and normal tissues is beneficial for early diagnosis of tumors.Methods:High-throughput sequencing was used to detect Serum miRNA in 10 HNSCC patients(including five cases of laryngeal carcinoma and five cases of hypopharyngeal carcinoma)and five normal patients.Five normal control subjects except basic diseases.Pathological diagnosis was conducted on samples from surgery.Results:A total of 23 differentially expressed miRNAs were identified to be upregulated in patients with laryngeal carcinoma,compared with the normal group.In the hypopharyngeal carcinoma,there were 12 differential miRNAs up-regulated and 7 differential miRNAs down-regulated,in comparison to the normal group.Conclusion:The key genes were further explored from the differentially expressed miRNAs identified from the laryngeal cancer group,the hypopharyngeal carcinoma group and the normal group.The key miRNAs up-regulated included hsa-miR-125b-5p,and the key miRNAs down-regulated were:hsa-miR-17-5p,hsa-miR-182-5p,hsa-miR-7-5p in the laryngeal cancer group and the normal group.In the hypopharyngeal carcinoma group and normal group,the key miRNAs up-regulated were:hsa-miR-125b-5p,hsa-miR-96-5p,hsa-miR-30a-5p,and the key miRNAs down-regulated included:hsa-let-7a-5p,hsa-miR-7-5p.