Mechanism Of Lycium Barbarum Polysaccharide On Apoptosis Of Mouse Bone Marrow Mononuclear Cells Induced By Radiation

Objective:Lycium barbarum is a Chinese herbal medicine,the main active ingredient of which,Lycium barbarum polysaccharide(LBP),has a variety of pharmacological effects.The purpose of this study was to investigate the effect of Lycium barbarum polysaccharides on apoptosis of mouse bone marrow mononuclear cells(BMNCs)induced by radiation,and to detect the inhibitory effect of different doses of Lycium barbarum polysaccharides on radiation-induced apoptosis and its possible mechanism.Methods:Mouse bone marrow mononuclear cells were cultured in vitro and divided into blank group(no radiation and LBP treatment),control group(radiation only),200 ?g/mL LBP(radiation+200 ?g/mL LBP),400 ?g/mL LBP(radiation+400 ?g/mL LBP),800 ?g/mL LBP(radiation+800 ?g/mL LBP).After 24 hours of LBP treatment,cell viability was detected by cck-8method,apoptosis rate was examined by flow cytometry(FCM),mitochondrial membrane potential fluorescence was detected by JC-1,and the expression of mitochondrial pathway-associated protein was measured by Western blotting.Results:The results showed that cell viability was significantly increased in the groups of 400 ?g/mL LBP and 800 ?g/mL LBP(P<0.01),and the early apoptosis rate of radiation-induced BMNCs was decreased in the groups of LBP(P<0.05)compared with the control group after 24 hours of LBP treatment.In a mitochondrial membrane potential fluorescence assay,LBP reduced the radiation-induced decrease in mitochondrial membrane potential(P<0.01).This was further demonstrated in a western blot analysis,in which LBP inhibited the mitochondrial release of cytochrome C and the expression of caspase 9,caspase 3,and PARP(P<0.05).Conclusion:Thus,LBP can inhibit the apoptosis of radiation-exposed mouse BMNCs,and the mechanism is likely to be achieved by inhibiting mitochondrial apoptosis.