Effect Of Taurine On Endoplasmic Reticulum Stress In Ins-1 Cell Lines Induced By High Fat And Glucose

Type 2 diabetes mellitus(T2DM)has now crossed the age limit and become one of the highest incidence of diabetes among humans,which is closely related to the diet with high sweetness and fat.Excessive intake of sugar and fat in the body can impair islet cell function and insulin resistance,leading to type 2 diabetes(li guangwei,2002).The initiation of apoptosis process of cells of islet is closely related to the development of diabetes mellitus.Taurine is an important free amino acid with multiple biological functions.Not only can T2 DM be prevented and treated by increasing insulin sensitivity,but also one of the effective methods is to protect the functional integrity of islet cells.The preliminary results of our research group showed that taurine could alleviate stress and inhibit the apoptosis of pancreatic islet cells by inhibiting the expression of key proteins in the ers-mediated apoptosis-related signaling pathway of ire1-caspase12.In this study,ins-1 cells of islet cell line treated with high fat and sugar were used as the research object to investigate the expression of key proteins in the ers-mediated CHOP apoptosis-related signaling pathway and JNK apoptosis-related signaling pathway induced by taurine,and to explore the mechanism of taurine.Test selection will INS-1 cells in vitro,and divided into four groups,respectively,blank control group(group C,1640 medium),taurine in the control group(T group,1640 + taurine),high fat and sugar group(group H,1640 + high fat and sugar),high fat and sugar taurine group(HT group,1640 + high fat and sugar + taurine),respectively,12 H,24 H and 48 H after training for testing.After culture for 12 h,changes in the expression of p-eif2,ATF6,IRE1 and XBP1 proteins were detected by immunofluorescence to detect the upstream factors of endoplasmic reticulum stress initiation,and the apoptosis-related signaling pathway mediated by taurine in the endoplasmic reticulum stress mediated ins-1 cells was explored by western blot.The expression levels of ATF6,PERK,p-PERK,p-eif2,eif2 a,CHOP,p-chop in the JNK signaling pathway were affected by the expression levels of p-jnk,JNK,XBP1,EDEM,Bcl-2,ASK1 and CHOP signaling pathways.Fluorescence detection results show that:(1)the taurine in the control group(T group)of XBP1,IRE1,p-eif2 alpha protein expression of the fluorescent detection results compared with the blank control group(group C)were downward trend(P< 0.001),taurine in the control group(T group)ATF6 protein expression of the fluorescent detection results compared with the blank control group(group C)were downward trend(P< 0.01),presumably,taurine can inhibit the endoplasmic reticulum stress occurred,the protective effect of islet beta cells.High fat and sugar model group(group H)of ATF6,XBP1,IRE1,p-eif2 alpha protein fluorescence detection results compared with the blank control group(group C)showed a trend of significantly increased(P< 0.001),but the taurine high fat and sugar group(HT group)with high fat and sugar than model group(group H)ATF6,XBP1,IRE1,p-eif2 alpha expression levels were significantly decreased(P< 0.001),indicating that received high fat and sugar in islet beta cells induced by endoplasmic reticulum stress occurs,taurine can be mediated by inhibition of endoplasmic reticulum stress related protein expression of apoptosis,Delay endoplasmic reticulum stress,inhibit islet cell apoptosis,prolong the life of damaged islet cells,and play a protective role.(2)Western Blot results showed that:cell culture 12 h,24 h,taurine in the control group(T group)in ATF6,XBP1,p-eif2 alpha group(T)protein expression level compared with the blank control group(group C)significantly lower(P<0.001),p-PERK,p-eif2 alpha,ATF6,XBP1,ASK1 protein expression level high fat and sugar group(group h)was significantly higher than that of blank control group(C group)(P < 0.001),taurine,high fat and sugar group(HT group),significantly lower than the high fat and sugar group(h)(P<0.001);The protein expression level of EDEM group(group H)was significantly higher than that of blank control group(group C)(P<0.001),and taurine group(HT group)was significantly lower than that of group H(P<0.001).after 48 h of cell culture,the apoptosis-related factors p-chop in er stress group were significantly higher than those in the blank control group(P<0.001),and taurine group(HT group)were significantly lower than those in h group(P< 0.001).Taurine in the control group(T group)compared with the blank control group(group C)apoptosis related factors of endoplasmic reticulum stress start p-JNK protein expression level difference was not significant(P > 0.05),high fat and sugar group(group H)was significantly higher than that of blank control group(C group)(P<0.001),taurine,high fat and sugar group(HT group)was significantly lower than the high fat and sugar group(group H)(P<0.01);The expression level of anti-apoptotic factor Bcl-2 protein in the high-fat and high-sugar group was significantly higher than that in the blank control group(P<0.001),and that in the high-fat and high-sugar taurine group(HT group)was significantly higher than that in the high-fat and high-sugar group(H group)(P< 0.01).In conclusion,high-fat and high-sugar environment can induce er stress and initiate apoptosis process.Taurine can alleviate er stress by inhibiting er stress related protein expression and increasing the expression level of anti-apoptotic protein,so as to inhibit er apoptosis and protect er cells.