Effects Of Chronic Treatment With Clostridium Butyricum On Bone Loss In A D-Galactose-Induced Aging Rat Model

Objective:To explore the protective effect of Clostridium butyricum on bone loss in male rats treated with D-galactose.Methods:Thirty-three three-month-old male rats were randomly divided into 3 groups:control group(Sham group),D-galactose treatment group(D-Gal group)and Clostridium butyricum treatment group(CB group),with 11 rats in each group.All rats were treated with intraperitoneal injection of d-galactose or normal saline.Clostridium butyricum group was given intraperitoneal injection of d-galactose and clostridium caseate gavage for 8 weeks.At the end of the experiment,rats were sacrificed to obtain serum and femur.The effectsof treatments were evaluated by biomechanical tests,Micro-CT scans and serum biochemical analysis.Results:Compared with untreated D-Gal group rats,the systemic administration of Clostridium butyricum elevated the levels of bone metabolic markers[type I procollagen amino terminal propeptide(PINP)and type I collagen carboxy terminal peptide(CTX-I)],increased bone mineral density(Tb.BMD),improved trabecular bone parameters in rats [trabecular bone volume fraction(BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th),and trabecular separation Degree(Tb.Sp)],all reached statistically significance(P<0.05).Conclusion:Clostridium butyricum can increase serum testosterone level and effectively reduce bone loss in male rats induced by D-galactose.