Effects Of Up-regulation Of MiRNA-147 Expression On Proliferation,Apoptosis,Cycle,Invasion And Migration Of Glioma U87 Cells

Objective:To determine whether there is abnormal expression of miRNA-147 in glioma tissue,and to study whether miRNA-147 can affect the proliferation rate,apoptosis rate,cell cycle percentage,invasion ability and migration activity of glioma cells.Methods:45 pairs of glioma tissues and adjacent normal tissues were collected,and the expression of miRNA-147 in 45 pairs of glioma tissues and adjacent tissues was detected by RT-QPCR.The difference of miRNA-147 expression between glioma tissue and corresponding peri-tumor tissue was statistically analyzed.Then the cell experiment was carried out,and the glioma U87 cells were transfected with Lipo2000 liposome method.MiRNA-147 mimics and NC were transfected into U87 cells respectively,and then divided into two groups,including miRNA-147 mimics group and NC group.after transfection,the transfection efficiency was judged by RT-QQPCR technique.On the basis of CCK-8,flow cytometry,transwell chamber and other reagents or instruments,the phenotypes of NC and miimics cells were analyzed.Results:45 pairs of glioma specimens were detected and found that the expression level of miRNA-147 in glioma tissue was lower than that in normal cells adjacent to glioma.In the experiment of cell proliferation detection,the results of 48 h and 72 h showed that the OD value of miRNA-147 mimics group was significantly lower than that of NC group,the difference was statistically significant.In flow cytometry,the apoptosis rate ofmiRNA-147 mimics group was significantly higher than that of NC group,the difference was statistically significant.the increase of late apoptosis in miRNA-147 mimics group was the main characteristic.In flow cycle test,the ratio of cells in G2/M phase in miRNA-147 mimics group was significantly higher than that in NC group,and the difference was statistically significant.In the migration detection test,the migration distance of cells in miRNA-147 mimics group was significantly lower than that in NC control group.In the thanswell chamber experiment,the number of cells penetrating the membrane into the lower chamber in the miRNA-147 mimics group was significantly lower than that in the NC group,and the difference was statistically significant.Conclusion:The expression of miRNA-147 was low in glioma tissues.Overexpression of miRNA-147 in U87 cells can significantly inhibit the proliferation,migration and invasion of U87 cells,block the normal cell cycle,increase the number of cells in G2/M phase,and limit their mitosis.At the same time,miRNA-147 could induce late apoptosis of U87 cells and increase the apoptosis rate of U87 cells.MiRNA-147 may play the role of tumor suppressor gene in glioma,and it can be used as a new marker for the diagnosis and treatment of glioma.