Preliminary Safety Experiment Of Dry Eye Hydrogel Traditional Chinese Medicine Eye Patch And Clinical Questionnaire Survey Of Dry Eye

Objective:By applying drugs to the skin around the eyes and back of guinea pigs,conjunctival congestion,corneal fluorescein staining,conjunctival impression cytology,and skin irritation reaction were preliminarily observed to evaluate the safety of liquid medicine gel eye patch in dry eyes and lay a foundation for further related research;The prevalence rate and high-risk factors of dry eye were investigated through questionnaires to provide reference data for futher development of related research on dry eye.Methods:1.Animal experimentsThirty-two healthy guinea pigs were randomly divided into 4 groups,namely blank group,low concentration group,high concentration group and skin group,8 in eachgroup.Unified baseline before experiment.Blank group:No intervention.Low and high concentration groups:Corneal fluorescein staining and conjunctival congestion index were taken on the 1st,4th,7th,11 th and 14 th days after medication,and conjunctival blotting cytology index was taken on the 14 th days and before medication.Skin group:At the 1h、1d、2d、3dafter medication,bilateral skin conditions on the back of guinea pigs were observed.The test cycle is 14 days.2.Questionnaire surveyFrom December 2018 to February 2019,158 patients were enrolled in the ophthalmology outpatient department of our hospital,and Understand their gender,age,educational level,refraction,computer use,contact lens wearing,bad living habits(smoking,drinking,staying up late),allergy history,etc.Statistical analysis was carried out according to the scores.Results:1.Animal Experiment Results(1)conjunctival congestion: There was no significant difference in Intragroup Comparison of Conjunctival Congestion Grading among the blank group,low concentration group and high concentration group with the increase of medicationtime.There was no significant difference between the groups.(2)Cornea fluorescein staining: There was no significant difference in the blankgroup between before and after the experiment.With the increase of medication time in low concentration group,there was no significant difference in Cornea fluorescein staining score.With the increase of medication time,the Cornea fluorescein staining scores of the high concentration group increased on the 7th,11 th and 14 th days compared with that before the experiment,and the difference was statistically significant.There was no significant difference between blank group and low concentration group at each time node.The scores of high concentration group were higher than those of blank group and low concentration group,and the difference was statistically significant.(3)Conjunctival blotting cytology: Before and after the experiment,there was no significant difference in conjunctival goblet of the blank group.Before and after medication,the difference of conjunctival goblet cells in low concentration group was not statistically significant.The difference of conjunctival goblet cells in high concentration group was statistically significant before and after medication.At the end of the experiment,the difference of conjunctival goblet cells between blank group and high concentration group,low concentration group and high concentration group was statistically significant and there was no significant difference in conjunctival goblet cells between blank group and low concentration group.(4)Skin irritation test: No skin congestion,redness,exudation,erosion and other manifestations occurred at each time node in the skin group.2.Results of Questionnaire SurveyA total of 158 valid questionnaires were included.Dry eye accounted for 41.1%.All risk factors were included in the single factor analysis model.After logistic analysis,the results showed that The risk of dry eye for people with bad living habits is 5.689 times higher than those with good living habits andLong-term use of computers increases the risk of dry eye by 30.843 times.Conclusion:1.The dry eye gel used in this experiment has no obvious irritation to the skin ofguinea pigs2.The low concentration dry eye TCM gel eye patch had good safety,and there is the possibility of damage to cornea and conjunctiva in high concentration dry eye TCMgel eye patch.It is suggested that drug concentration and duration are potential risk factors that need attention.3.This questionnaire survey shows that bad living habits and computer use may be high risk factors for dry eye.