Research On The Mechanism Of Androgen Receptor-associated Enhancer RNA EMARC1 Involved In DHT Regulation Of Bladder Cancer Cell Growth

Background:Bladder cancer is the most common malignant tumor of the urinary system in the world,especially in elderly men.It has been reported that men are four/five times more likely than women to develop bladder cancer,which contains androgen receptor(AR)in the tissue and is a family of hormone-associated tumors.Studies have shown that androgen and its receptor pathway play an important regulatory role in the occurrence and development of bladder cancer.Enhancer RNA is a kind of non-coding RNA produced by enhancer transcription,which can activate the activity of enhancers and promote the expression of target genes.Previous studies have found that enhancer RNA plays an important role in many diseases such as tumor,especially androgen receptors related enhancer RNA,which are closely associated with prostate cancer,showed that the correlation between tumor may be a sex hormone kind disease factor,its mechanism about the progress of the bladder cancer remain unclear,which need further exploration.This study will explore the biological role and potential function of androgen and androgen receptor-associated enhancer RNA eMARC1 in the occurrence and development of bladder cancer.Methods: The relative expression of eMARC1 was detected by real-time fluorescence quantitative PCR(RTq-PCR)in 37 bladder cancer clinical tissue samples,and its correlation with disease classification and staging was analyzed.Artificial micro RNA interference was used to knock down the expression level of eMARC1 in T24 and 5637 bladder cancer cells,and DHT was used to stimulate and up-regulate the expression level of eMARC1 in T24 and 5637 bladder cancer cells.Cell proliferation,migration and apoptosis were detected.Result: eMARC1 was highly expressed in bladder cancer tissues and cell lines(P<0.01),and the expression level of eMARC1 was closely related to tumor stage(P=0.008).Artificial micro RNA can effectively knock down the expression level of eMARC1,thereby inhibiting the proliferation and migration of bladder cancer cells and promoting the apoptosis of bladder cancer cells.The expression level of eMARC1 was significantly increased after DHT treatment,which can promote cell proliferation and migration and inhibit apoptosis.It was further found that the down-regulation of eMARC1 reduced the androgen effect of DHT in bladder cancer cells.Conclusion: eMARC1 plays an important role in bladder cancer cell progression.DHT may promote the development of bladder cancer by activating eMARC1.eMARC1 may be a new therapeutic target which improve the therapeutic effect of bladder cancer.