| Objective This study investigated the effect of diabetes mellitus on the reconstruction of anterior cruciate ligament(ACL)in the diabetic rabbit induced by alloxan(ALX)and evaluated the effectiveness of the experimental model.Methods Thirty-six healthy male New Zealand rabbits were randomly divided into two groups,A and B.They were fasted 12 hours before injection via auricular vein route.Group B was given with two doses of ALX at the concentration of 80 mg/kg and 120 mg/kg at an interval of 48 h.Group A was injected with equivalent amount of physiological saline in the same manner as controls.Weight and fasting blood glucose monitoring combined with immunofluorescence detecting,histological evaluation were carried out during the experiment period.After harvest of the stable animal models of diabetic rabbits,ACL reconstructions were performed in all rabbits under general anesthesia.Each tendon graft was harvested from the lateral third of the Achilles tendon.After excision of the ACL,tibial and femoral bone tunnels were created to implant each graft in the native ACLposition.Gross observations combined with bone mineral density(BMD)detecting,histological evaluation,real-time polymerase chain reaction(RT-PCR)testing,magnetic resonance imaging(MRI)and micro computed tomography(Micro CT)scanning were performed after 2,4 and 8 weeks.Results The fasting blood glucose concentration of the group B was higher than 300 mg/dL during the experiment period,and that was significantly higher compared with group A(P<0.01).Gross observation showed that group A had smooth surface and homogeneous texture of the tendon graft in the articular cavity,while group B had rough surface,brittle texture,and yellow color of the tendon graft.Our results indicated that there was no difference in BMD of the bone tunnel between the two groups at 2 weeks after surgery.But at 4,8 weeks after surgery,the BMD of group B was significantly lower compared to group A(P<0.01).Similarly,the width of tibial bone tunnel in group B was significantly larger than group A(P<0.01)via MRI at 4,8 weeks after surgery.Through Micro CT,the average tibial bone tunnel area in group A was significantly smaller than group B(P<0.05),but the bone volume/ total volume(BV/TV)of tibial bone tunnel was significantly higher compared with group B(P<0.05)at 4,8 weeks after surgery.Histologically,at 8 weeks after surgery,the pancreas of group B showed marked beta cells damage and the decrease in numbers.The degenerative kidney tubules were found and the hyaline casts were also seen in group B at 8 weeks after surgery.Additionally,compared togroup B,the fibrous tissue in the interface of tendon-bone area was more ordered and denser in group A at 4,8 weeks after surgery.At the same time,the degree of maturity in new trabecular bone from group A was higher than group B.Conclusion 1.This is a reliable,efficient,stable animal model of ACL reconstruction in the alloxan-induced diabetic rabbit.2.The changes in the structure of tendon-bone interface from diabetic rabbit result in the adverse effects on the growth of tendon-bone healing. |
PDF Full Text Request