Analysis Of Vesicle Death And Its Causes After Co-culture Of Echinococcosis And Hepatocarcinoma Cells

Objective:To study the death pattern and cause of the cochlear culture of different types of hepatocellular carcinoma cells in vitro,and to explore the effect of feeder cellconcentration on the survival rate of the original head lice.method:The protoscolex of alveolar hydatid was obtained from the abdominal cavity of the mice,which were dissected and fed for more than 6 months.The protoscolex of alveolar hydatid was co-cultured with different numbers of feeder cells in the D-MEM medium.According to the concentration of feeder cells,the experiment consisted of four experimental groups,each group was fed with DMEM medium,2000 protoscoleces,of which one group was blank control group without feeder cells;two groups were 1*10^ 6hepatocellular carcinoma cells;three groups were 2*10^ 6 hepatocellular carcinoma cells;four groups were 3*10^ 6 cells to count hepatocellular carcinoma cells.The culture medium and feeder cells were replaced once a week and cultured in a 37 C 5% C02 incubator for 3 weeks.During the experiment,the growth of protoscolex was observed weekly,and the mortality of protoscolex was recorded.Result:The survival rate of protoscolex cultured in blank group was significantly different from that in three experimental groups(p < 0.05).The survival rate of protocercariae cultured in three experimental groups with feeding cells was not significantly different in the first week.The majority of protoscolex in blank group died in the second week,and protoscolex existed in the third group of three experimental groups(p < 0.05).The highest survival rate was found in both groups(p < 0.05).There was no significant difference in survival rate between groups 2 and 4.In the third week,the survival rate of protoscolex in the blank group was basically stable,and there was statistical difference between the second group and the fourth group(p < 0.05).The survival rate of the second group was significantly different from that of the fourth group(p < 0.05).The survival rate of group 3 was better than that of other experimental groups and control groups.Conclusion:1.Mouse hepatocarcinoma cells are conducive to the survival of protoscolex cultured in vitro;the survival rate is closely related to the number of feeder cells;2.High cell volume feeder cells can lead to protoscolex death in the later stage;3.The survival rate of hepatocarcinoma feeder cells in mice with low cell number is better than that in mice with high cell number.