| Objectives The purpose of this study was to explore the feasibility of using the arterial clamp to manufacture the acute spinal cord injury(ASCI)model in male Sprague-Dawley(SD)rats,and to inject a large amount of rhodiola in the abdominal cavity into the model.The effects of Rhodiola rosea on the kinematics,oxidative stress and the recovery of nerve cells were studied.Methods Thirty-six 2-month-old male SD mice(200g ±10g)were randomly divided into three groups: control group(sham group,n = 12),model group(SCI group,n = 12)and experimental group(n = 12,n = 12).Sham group,SCI group and administration group received bilateral laminectomy(T10 vertebra),respectively.The T10 vertebrae in the SCI group and the administration group were given the same height of spinal cord for 3 seconds.After operation,the large-strain Rhodiola injection(10mg/ kg ·d),SCI group and sham group was injected intraperitoneally with the same amount of normal saline,and the same amount of saline was injected intraperitoneally into the drug group after operation.On the 3rd day,6 rats in each group were killed at random and the related oxidative stress indexes were measured.The remaining 6 rats in the administration group continued to be administered until the 7th day,and the remaining rats in the SCI group and the sham group continued to receive the same amount of saline injection.All the spinal cord specimens were perfused with 4% paraformaldehyde in vivo until the 14 th day in the standard environment.The samples were immersed in 20% sucrose solution for 24 hours(at 4?)and dehydrated until the tissue was completely submerged.The dehydrated specimens were embedded in frozen sections to observe the injury and recovery by he staining and to detect the survival and recovery of nerve cells by immunohistochemical staining.Improved Tarlov scores were performed before operation(0 days)and 1,3,5,7,10,14 days after operation to evaluate the recovery of hind limb movement.The data of each group were collected for statistical analysis.Results 1.Compared with sham group,SCI group showed swelling,necrosis,apoptosis,vesicular cavity,structural disorder of regenerated axon,increased number of glial cells,and unclear boundary between gray matter and white matter.The modified Tarlov score decreased significantly(P<0.05).Oxidative stress indexes showed that the content of superoxide dismutase(SOD)(P<0.05),glutathione(GSH)(P<0.05)and malondialdehyde(MDA)increased significantly(P<0.05),and the content of malondialdehyde(MDA)increased significantly(P<0.05).The content of nitric oxide(NO)increased slightly(P> 0.05).Immunohistochemical staining showed that the number of neurons labeled with neurofilament protein 200(neurofilament protein 200,NF 200)decreased significantly(P < 0.05),the growth associated protein 43(GAP-43)labeled axon regeneration structure was disordered,and the number of ineffective hyperplasia increased.2.After the intervention of large-strain Rhodiola injection,compared with the SCI group,the swelling of nerve nucleus in the injured area was improved obviously,the cystic cavity was reduced,the regenerated axon structure was orderly,the mutual support and the ineffective scar were reduced in the treatment group.The improved Tarlov score was significantly increased(P<0.05),and the oxidative stress index showed that the content of superoxide dismutase(SOD)and glutathione(GSH)were significantly increased(P<0.05)and GSH(P<0.05),respectively.MDA content decreased significantly(P<0.05),nitric oxide(NO)content decreased slightly(P>0.05).Immunohistochemical staining showed that the number of neurons labeled with neurofilament protein 200(neurofilament protein 200,NF 200)was significantly increased(P<0.05).The structure of astrocytes labeled with growth associated protein 43(GAP-43)was clear and ineffective proliferation was significantly decreased.Conclusions Rhodiola injection can improve the secondary oxidative stress response induced by sci,promote the recovery of motor function of hind limbs of sci model,reduce the apoptosis of neuronal edema in the injured area,and effectively induce axon remodeling.Promote the recovery of nerve cells.Figure 5;Table 6;Reference 117... |
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