Effects Of Gancao Xiexin Decoction On Mycelial Diversity In Mice With Intestinal Mycelial Disorder

Objective Study on the effect of different doses of Gancao Xiexin decoction on the expression regulation of s Ig A in intestinal flora and intestinal tract.By using antibiotics to establish the model of intestinal flora disorder,using Gancao Xiexin Decoction to intervene,studying the effect of Gancao Xiexin Decoction on the internal environment of intestinal flora,The diversity of intestinal flora in mice treated with Gancao Xiexin Decoction for 14 days was measured by 16 Sr DNA sequencing,preliminary study on the mechanism of licorice decoction on intestinal flora and provide scientific basis for clinical application.Methods The 72 healthy experimental male mice were randomly divided into 6groups,namely the normal group,the model group,Gancao Xiexin Decoction high dosage group,Gancao Xiexin Decoction middle dosage group and Gancao Xiexin Decoction low dosage group,and the western medicine treatment group In addition to normal group given saline,the remaining 5 groups were given Lincomycin hydrochloride to establish a model of intestinal flora disorder after 7 consecutive days of gastric irrigation.After the success of the model,Chinese medicine were given the corresponding dosage of Gancao Xiexin Decoction,and the western medicine group were given the same dosage of Bifidobacillus living tablets to the Chinese medicine group.the normal group and recovery group were given isodose physiological saline,course of 14 days.After treatment,collection of feces from the intestinal tract of mice,collect the sample genomic DNA,then PCR amplification of 16 Sr DNA of intestinal microorganisms in mice with specific primers,to analyse the change of intestinal flora diversity in mice.Results 1.bifidobacterium:The number of OTUs sequences in Gancao Xiexin Decoction middle dosage group is significantly higher than model group,and higher than the Gancao Xiexin Decoction high dosage group,the Gancao Xiexin Decoction low dosage group,and the western medicine treatment group.That is,species abundance is higher than that of the Gancao Xiexin Decoction high dosage group,the Gancao Xiexin Decoction low dosage group,and the western medicine group.2.Lactobacillus:The number of OTUs sequences in Gancao Xiexin Decoction middle dosage group is significantly higher than model group,and higher than the Gancao Xiexin Decoction high dosage group,the Gancao Xiexin Decoction low dosage group,and the western medicine treatment group.That is,species abundance is higher than that of the Gancao Xiexin Decoction high dosage group,the Gancao Xiexin Decoction low dosage group,and the western medicine treatment group.3.Relative abundance of species in the normal group > Relative abundance of species in the Gancao Xiexin Decoction middle dose group>Relative abundance of species in the western medicine group > Relative abundance of species in the Gancao Xiexin Decoction high dose group= Relative abundance of species in the Gancao Xiexin Decoction low dose group>Relative abundance of species in the model group.4.The shannon index in the normal group >the Gancao Xiexin Decoction middle dosage group > the Gancao Xiexin Decoction high dosage group > the Gancao Xiexin Decoction low dosage group > the western medicine treatment group > the model group.5.For Beta Diversity Analysis,Comparison with the normal group,the dissimilarity index of the Gancao Xiexin Decoction high dosage group is 0.141;the dissimilarity index of the Gancao Xiexin Decoction middle dosage group is 0.118;the dissimilarity index of the Gancao Xiexin Decoction low dosage group is 0.129;the dissimilarity index of the western medicine treatment group is 0.172;the dissimilarity index of the model group is 0.291.Conclusion 1.Regulation of probiotics in the intestine(bifidobacterium?Lactobacillus),the the Gancao Xiexin Decoction high?middle?low dosage group and the western medicine group,both have a certain improvement effect,the Gancao Xiexin Decoction middle dosage group was better than the Gancao Xiexin Decoction high and Gancao Xiexin Decoction low dosage group and the western medicine group.2.The the Gancao Xiexin Decoction high?middle?low dosage group and the western medicine group can improve the diversity of intestinal flora,the Gancao Xiexin Decoction middle dosage group was better than the Gancao Xiexin Decoction high and Gancao Xiexin Decoction low dosage group and the western medicine group.