| Objective: To investigate the effects of intake of different probiotics and dietary components on postoperative recovery and irritant dermatitis around ileostomy of ileostomy in rats.To provide experimental research basis for diet education and stoma nursing of postoperative patients with ileostomy in clinical practice.Mrthods: 30 male Sprague wrote-Dawley(SD)rats,weighting 340-380 g,were randomly divided into Saccharomyces boulardii,(S.boulardii)group,Lactobacillus acidophilus,(L.acidophilus)group,High fat diet(HFD)group,Soluble dietary fiber(SDF)group.All rats underwent ileostomy procedure.Intervention was conducted from the 3rd day after the operation.The weight,food and water intake and feces of the rats were recorded daily.At the 10 th day after the operation,1ml ink was given to the stomach.Intestinal propulsion ratewas calculated by measuring the length of ink in small intestine and the length of pylorus to ileostomy 10 minutes later,Blood samples were collected for determination of serum Albumin(ALB)and Prealbumin(PA)levels.Fecal samples were collected and the changes in intestinal microbiome of rats were detected by high-throughput sequencing.To evaluate the severity of peristomy irritant dermatitis.Skin samples with dermatitis were collected and stained with HE to observe the depth of infiltration.After homogenized tissue,the concentration of TNF-αwas detected by enzyme-linked immunosorbent assay.SPSS22.0 was used for statistical analysis of the results.One-way anova was used for comparison between groups.Results: 1.General condition:(1)hair,activity and mental state: the dorsal hair of the rats in each group was less shiny and slightly messy,with acceptable mental state,less activity than before,and still sensitive response in all postoperative rats.(2)food and water intake: the daily intake and water intake of rats in each group fluctuated,and tended to level off on the 7th day after surgery;There was no significant difference between the two groups(P > 0.05).2.Nutrition status:(1)body weight: there was no significant difference in preoperative weight between the groups.Postoperatively,the weight of rats in each group decreased.On the 10 th day after surgery,the weight of control group was smaller than that of S.boulardii group and L.acidophilus group,and the difference was statistically significant(P < 0.05).The weight of the HFD group was higher than that of the control group,and the difference was statistically significant(P < 0.05).The body weight of SDF group was higher than that of control group,and the difference was not statistically significant(P > 0.05).(2)ALB and PA:the plasma ALB and PA levels of S.boulardii group and L.acidophilus group were higher than those of the control group on the 10 th day after surgery,and the difference was statistically significant(P < 0.05).ALB and PA levels in HFD group were higher than those in SDF group and control group,and the difference was statistically significant(P < 0.05).Serum ALB and PA levels in SDF group were lower than those in control group,and the differe nce was not statistically significant.3.Intestinal propulsion rate: the intestinal propulsion rate of the S.boulardii group was lower than that of the control group,and the difference was statistically significant(P < 0.05);S.boulardii group was slightly lower than that of the L.acidophilus group,but the difference was not statistically significant(P > 0.05).L.acidophilus group showed no significant difference from the control group(P < 0.05).HFD group was higher than that of SDF group,and the difference was not statistically significant(P > 0.05).There was no significant difference between the HFD group and the SDF group and the control group(P > 0.05).4.Fecal evaluation:(1)Bristol feces score:Thefaecal score of S.boulardii group was lower than that of control group,and the difference was statistically significant(P< 0.05).The L.acidophilus group was lower than the control group and the difference was statistically significant(P< 0.05).The faecal score of HFD group was lower than that of control group,and the difference was significant(P< 0.05).The faecal score of SDF group was lower than that of control group,and the difference was significant(P< 0.05).(2)intestinal microbiome evaluation: the abundance of intestinal microbiome in each group was changed in phylum level.The relative abundance of Firmicutesand Bacteroidetesin S.boulardii group and L.acdophilus group was higher than that in the control group,and the relative abundance of Proteobacteria and Actinobacteriawas lower than that in the control group.The relative abundance of Firmicutes and Proteobacteria in the HFD group was higher than that in the control group,and the relative abundance of Bacteroidetes and Actinobacteria was lower than that in the control group.The relative abundance of Firmicutes,Proteobacteria and Bacteroidetes in the SDF group were higher than those in the control group,and the relative abundance of Actinobacteria was lower than those in the control group,but the differences were not statistically significant(P > 0.05).5.Peristomal irritant dermatitis: irritant dermatitis was observed in all rats in each group.The concentration of TNF-αin tissue samples of S.boulardii group and L.acidophilus group was lower than that of control group,and the difference was statistically significant(P<0.05).HFD group was higher than the control group,and the difference was no statistically significant(P>0.05).HFD group was lower than SDF group,and the difference was no statistically significant(P>0.05).There was significant difference between SDF group and control group(P<0.05).Conclusions: The model of ileostomy procedure in rats was established by microsurgery,which can be used for subsequent studies on postoperative recovery and peristomal irritant dermatitis.(2)Intake of probiotics can improve intestinal barrier function,bychanging the abundance of intestinal microbiome in the terminal ileum.The changing fecal characters can improve postoperative nutritional status by increasing the absorption of nutrie nts and reduce the severity of irritant dermatitis around the stoma and help postoperative recovery at the same time.(3)Dietary components such as fat and dietary fiber intake can also change the structure and composition of intestinal microbiome in terminal ileum,leading to postoperative malnutrition,causing changes in ileostomy contents in rats,aggravating the occurrence of irritant dermatitis around the stoma and affecting postoperative recovery.(4)Postoperative probiotics addition and diet control can effectively improve postoperative status and severity of irritant dermatitis in rats,which provides objective experimental research basis for the nursing of patients after ileostomy. |
PDF Full Text Request