Diagnosis Of Sudden Cardiac Death By Detecting MiRNA Levels Forensic Experimental Study Of Myocardial Infarction

OBJECTIVE Sudden cardiac death(SCD)is mainly an accidental,rapid natural death characterized by a sudden loss of consciousness due to the heart itself.The main cause of SCD is acute myocardial infarction(AMI).At present,microRNAs have been confirmed by zoological experiments to diagnose AMI(acute myocardial infarction),and microRNAs still have important value in the diagnosis of acute myocardial infarction after different(PMI)AMI interval.The purpose of this paper is to explore the correlation between miRNA-1,miRNA-126,miRNA-133,miRNA-499 and PMI,AMI,and then to further study whether miRNA-1,miRNA-126,miRNA-133,miRNA-499 can be the basis for AMI-related sensitive biomarkers and PMI inferences.Therefore,the research on AMI-related factors is raised to the level of molecular biology,specificity and timeliness of markers related to myocardial discrimination diagnosis of acute myocardial infarction are further improved.Furthermore,it can effectively help forensic workers to quickly and accurately determine the cases of difficult AMI deaths and improve the authority of their appraisal opinions.METHODS First,the 56 surviving animals were divided into 4 time points,with 12 rats for each infarction.Then choose randomly four animals undergoing thoracotomy but not coronary ligation served as controls.PMI group: Rats were anesthetized and sacrificed for 0h,3h,6h,12 h and 24 h respectively.After the thoracotomy,the right ventricular blood was collected from the rats with blood collection needles,12 rats at each time point.PMI control group: 5 rats,rats were directly anesthetized and the right ventricular blood was collected by thoracotomy.Second,after ligating the left anterior descending coronary artery of the rat,the physiological signal acquisition and treatment system and HE staining were used to evaluate whether the acute myocardial infarction model was successful.Third,qRT-PCR was used to detect miRNA-1,miRNA-126,miRNA-133 and miRNA-499 in the heart of rats at 0.25 h,0.5h,1h and 2h after acute myocardial infarction Expression,which was introduced as a parameter into the regression equation to demonstrate the link between microRNAs and cases of sudden cardiac death caused by AMI.The same method was used to detect the content of miRNAs in rats at 0h,3h,6h,12 h,and 24 h after PMI.It proved that microRNAs can be used as arguments for PMI inference.Conclusions In this experiment,the difference between the control group and the experimental group for 0.25 h after acute myocardial infarction was first discussion.MiRNA-1,miRNA-126 h,miRNA-133 and miRNA-499 in the blood of rats were significantly higher than the control group.So there was a significant correlation and correspondence.It confirmed fhem are closely related to the occurrence of rat AMI,and predicted them can become a new biomarker for the diagnosis of AMI,which lays a solid foundation for the analysis of the death mechanism of SCD caused by early AMI in forensic work and the early treatment of patients with AMI.At the same time,the relationship between miRNA-1,miRNA-126,miRNA-133 and miRNA-499 and PMI was studied.The content of the four was stable within 12 hours after death,which has guiding significance for post-mortem PMI inference in forensic work.