The Study On The Nuclear Factor-κB/Collagen Ⅰ Expression Of Sudden Death In The Early Stage Of Acute Myocardial Ischemic.

Background: Most of sudden cardiac death was caused by acutemyocardial ischemia; multitudinous acute myocardial ischemiaresulted myocardial infarction and gynogenic sudden cardiac death(sudden manhood death syndrome, death from myocardial inhibition).Advanced stage myocardial infarction or old myocardial infarctioncould be diagnosed by focus of Infarct. To early myocardialischemia, it still depend on morphological changes been seen byroutine histopathology. Early myocardial ischemia is emphasis tostudy and research in forensic pathology. Search more sensitivediagnostic criteria is urgently required in medico legal research.Nuclear factor-kappa B (NF—κB) general reside in escargot, itis hinge of signal's trams in vascular endothelial cell, vascularsmooth muscle cell, cardiac muscle cell, and participated inpathos-alter. CollagenⅠreside in myocardial intercellularsubstance , it is one of the significant moieties, strangelyanti-tension, insulation. Could sustain, conservation,lubrication, restrict cardiac muscle cell. CollagenⅠis andynamic synthesize and degradation system, collagenⅠis the most in myocardial mesenchyma and participates early myocardialischemia and myocardial infarction. This investigation detectCollagenⅠand NF-κB in myocardium of Sudden cardiac deathcases by Immunohistochemical staining and observe changes ofischemia myocardium with the purpose of offer diagnostic evidencefor early myocardial ischemia.Purchases: To investigate the change of Nuclear factor-kappa B andCollagenⅠexpression the sudden cardiac death and to look for asensitive marker the diagnosis of early myocardial ischemia,Methods: Data was collected from 25 autopsy cases of sudden cardiacdeath in forensic medical Identification center of west chinaSichuan between 1999 and 2006. Filed paraffin blocks of the autopsyhave been divided into 3 groups according to morphologicalalteration by light microscope observation: control group, earlymyocardial ischemia groups and myocardial infarction group. By HEstain, Specific stain, Immunohistochemical staining, the positivestaining of NF-κB and collagenⅠin the myocardial nuclei ofdifferent group was observed, and semi quantitative analyses theseresults by Axiotis standard and SPSS 12.0.Results: Lie' s hematoxylin basic fuchsine picric acid: The yellowcolor was shown in ischemic area; ruby-red was shown in normalarea.Immunohistochemical staining of NF—κB: dispersive positivestaining of NF—κB in early myocardial ischemia groups andmyocardial infarction groups, and magnitude of express close toeach other, control group has no dispersive positive staining.Immunohistochemical staining of: In the control group, all thecollagenⅠwere connected into complex net. In the myocardialinfarction group, collagenⅠhave almost been disappeared; In the Ischemia group, collagenⅠfibers were broken, obscured in shape,in some area collagenⅠfibers were loosen and in disorder on thetransverse section and longitudinal section,.Conclusions: Immunohistochemical staining of nuclearfactor-kappa B/collagenⅠmay be a sensitive marker forpostmortem diagnosis in the early stage of acute myocardialischemia.