The Effects Of RNA Interference Of PSA Expression On The Cancer Cells Proliferation And Invasion In Human Prostate Cancer 22RV1 Cells

Objective To investigate the effects of Prostate Specific Antigen(PSA)on the proliferation,invasion and apoptosis of human castration-resistant prostate cancer(CRPC)22RV1 cells.To provide more evidences for supporting the potential therapeutic target of PSA for CRPC.Methods PSA-targeted shRNA lentivirus vectors containing shPSA1,shPSA2,sh PSA3,sh PSA4 or negative control(NC)were developed,and then were packaged in lentiviral particles,which were then used to infect castration-resistant prostate cancer 22RV1 cells for the generation of sh PSA1-,sh PSA2-,sh PSA3-,sh PSA4-,or NC-22RV1 cells respectively.The expression levels of PSA m RNA and protein were d measured by real-time fluorescent quantitative-PCR(q RT-PCR)and Western blot,respectively.22RV1-derived cells in which PSA was efficiently knocked down were chosen out.The proliferation,invasion abilities and apoptosis of 22RV1 cells were evaluated by CCK-8 assay,Transwell cell invasion assay and flow cytometry respectively.Results The expression levels of PSA m RNA were downregulated in sh PSA1-22RV1,sh PSA2-22RV1,and especially sh PSA3-22RV1 cells which showed the most significantly gene silencing efficacy(P < 0.01).The expression level of protein was significantly lower in sh PSA3-22RV1 cells than in NC-22RV1 cells(P < 0.01).The proliferation abilities of sh PSA2-and sh PSA3-22RV1 cells at 24,48 and 72 h after adherence were obviously inhibited as compared with those of NC-22RV1 cells(all P < 0.01);the invasion ability of sh PSA1-,sh PSA2-and sh PSA3-22RV1 cells were all decreased(P < 0.01).Cell apoptosis analysis by cell cytometry showed that the transfection of sh PSA2 and sh PSA3 construct increased the proportion of apoptotic cells(P < 0.01).Conclusion PSA promotes CRPC 22RV1 cell proliferation,invasion,and inhibites its apoptosis.In addition,the effect of PSA on Pr Ca is dose-dependent.