| Objective: This study evaluated the diagnostic efficacy of circulating miRNAs as a diagnostic marker for gastric cancer;Through database analysis and literature research,miR-26 a was used to determine the migration and invasion ability of gastric cancer.Targetscan and other databases were used to predict the target gene as IRTKS.At the same time,explored the effects of IRTKS on the migration and invasion of gastric cancer cells;elucidated the role of IRTKS in the metastasis of gastric cancer,and then elucidated the mechanism of gastric cancer development and the diagnosis of gastric cancer.Treatment provides new ideas.Methods: The research content is divided into two parts.The first part mainly uses the Meta-analysis method to analyze the diagnostic efficacy of circulating miRNAs in patients with gastric cancer.A literature search was first conducted to collect potentially eligible studies published by the self-built library until November10,2017 by searching online databases such as PubMed,Embase,Cochrane Library,Web of Science,and Springer.According to the inclusion and exclusion criteria of the plan,the literature was selected and extracted,including: first author,country,publication year,miRNAs test sample type,case group and control sample size,case group and control group mean age,curve Area and its 95% confidence interval,sensitivity and specificity.The QUADAS-2 checklist in Revman 5.0 software was used for quality evaluation,and Meta-Disc 1.4 software was used for heterogeneity test.Meta-regression analysis was used to investigate the source of heterogeneity factors,and then subgroup analysis and sensitivity analysis.Using the Gene Expression Omnibus public database to analyze the sequencing data of the miRNAs included in the study,it was found that miR-26 a showed a low expression trend in gastric cancer tissues and serum.Differential expression is meaningful.Throughliterature investigation,we found that miR-26 a overexpression significantly inhibited the migration and invasion of gastric cancer cells.We predicted that the target gene might be bound by IRTKS through Targetscan,miRDB,miRWalk and miRTarbase databases.The second part focuses on the ability of IRTKS to affect the migration and invasion of gastric cancer cells.The IRTKS overexpression and inhibition system was constructed and transfected into gastric cancer cell lines(SGC-7901,HGC-27).Transwell assay was used to analyze the effect of IRTKS overexpression and inhibition on the migration and invasion of gastric cancer cells(SGC-7901,HGC-27).Results: The first part: A total of 48 literatures containing 77 miRNAs were included in the analysis.The results showed that the sensitivity of circulating miRNAs to gastric cancer diagnosis was 0.76 and the specificity was 0.81.In addition,subgroup analysis showed that multiple miRNA combination analysis was based on single miRNA.Serology has a good diagnostic power for gastric cancer.GEO database analysis found that miR-26 a was down-regulated in gastric cancer tissues and serum and affected the migration and invasion ability of gastric cancer cells.The literature research combined with the database predicts that the target gene that may be bound is IRTKS.The second part: the IRTKS overexpression/knockdown system was constructed,and the IRTKS up-regulation/down-regulation experiment was carried out.It was found that over-expression of IRTKS can enhance the migration and invasion ability of gastric cancer cell lines(SGC-7901,HGC-27).Conclusion: Circulating miRNAs have important value in the diagnosis of gastric cancer.IRTKS can affect the migration and invasion of gastric cancer cell lines(SGC-7901,HGC-27). |
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