2-Deoxy-D-Glucose Rescues The Self-renewal Of Embryonic Stem Cells Inhibited By Sevoflurane

Objective:To assess the effects of sevoflurane on self-renewal of ESCs and determine whether the effects of sevoflurane on ROS level could be rescue by 2-DG.Methods:In this study,murine embryonic stem cells(mESCs)were used as a model to study the toxicity of sevoflurane to the first trimester embryo.mESCs were randomly divided into 4 groups: control group,sevoflurane group,2-DG group,and 2-DG + sevoflurane group.After 6 hours of treatment with 4.1% sevoflurane,the effects of sevoflurane on ROS expression and self-renewal of mESCs were observed in the following four aspects: the morphological changes of cells,the intracellular ROS level measured by fluorescence microscopy,the mRNA expression of the embryonic stem cell related gene detected by qRT-PCR and immunofluorescence staining,respectively.Result:Compared with the control group,the ROS level in the sevoflurane group was significantly higher(P<0.05),and the expression of embryonic stem cell related genes were decreased(P<0.05).Compared with the sevoflurane group,the ROS level in the sevoflurane+2-DG group was lower(P<0.05),and the expression of embryonic stem cell related genes were increased(P<0.05).Conclusion:Sevoflurane induced a decrease in the self-renewal capacityof mESCs by inducing intracellular ROS production.2-DG attenuates the high levels of ROS and reverses its ability to inhibit the self-renewal of embryonic stem cells.Our study has provided new insights into the toxic effects and mechanisms of sevoflurane-induced fetal development in the fetus,as well as in the prevention and treatment of embryotoxicity associated with inhalation anesthesia.