Relationship Between CD28 Rs3116496 T?C Polymorphism And Susceptibility To Esophageal Squamous Cell Carcinoma And Non-small Cell Lung Cancer

Objective: The aim of this study is to explore the relationship between CD28 gene rs3116496 T>C polymorphism and susceptibility to esophageal squamous cell carcinoma and non-small cell lung cancer.Methods: We conducted two hospital-based case-control studies.The first part included a case group of 829 patients with esophageal squamous cell carcinoma(ESCC)and a control group of 1522 non-tumor populations;The second part consisted of a group of 1193 patients with non-small cell lung cancer(NSCLC)and a control group of 1056 non-tumor populations.We collected venous blood from each subject and then extract DNA from the blood sample.SNPscan was used to detect the genotype of CD28 rs3116496 T>C in all specimens.Then statistical analysis was performed on the difference in the frequency of distribution between the CD28 rs3116496 T>C genotype between the case and the control group.Logistic regression was used to analyze the association between risk of ESCC and NSCLC and CD28 rs3116496 T>C polymorphism on overall comparison and stratificated analysis according to risk factors(e.g.age,sex,body mass index,smoking,and drinking).Results: Statistical analysis results show that(1)there was no significant difference in age and gender between the ESCC group and the control group(P=0.328,P=0.331).There was no significant difference in age and gender between the NSCLC group and the control group(P=0.960,P=0.425);(2)the genotyping success rate of all samples in this study was over 95%.The CD28 rs 3116496 T>C genotyping rate in the control group was consistent with the Hardy-Weinberg equilibrium;(3)smoking,drinking,body mass index in the esophageal squamous cell carcinoma group and its control group were statistically different,P value <0.001.There was also a statistical difference between NSCLC and control group,P value <0.001;(4)there was no significant difference in the genotype distribution of CD28 rs3116496 T>C locus between ESCC/NSCLC and controls,P value was greater than 0.05;(5)stratified analysis by different age,sex,body mass index,smoking,and drinking status showed that the difference was not statistically significant. Conclusion: In the development of ESCC and NSCLC,the polymorphism of CD28 rs3116496 T>C locus may be irrelevant.However,this conclusion needs to be studied again in different races,regions and more risk factors.