Study On Serum Metabolic Markers Of Essential Hypertension Complicated With Hyperuricemia Based On UHPLC/Q-TOF-MS

Background: Essential hypertension(EHT)is a common chronic disease and can cause damage to multiple target organs such as heart,brain and kidney.EHT combined with hyperuricemia(HU)further aggravates organ damage.Epidemiological studies have found that EHT populations have elevated blood uric acid levels and a higher incidence of HU than healthy people.Methods: The main objective of this study was to compare serum metabolites in EHT patients with EHT+HU patients and to find metabolic markers and related pathways in the progression of EHT to EHT+HU.Results: Thirty-five patients with EHT+HU were enrolled in Beijing Hangxing Hospital and their serum samples were collected for EHT and EHT+HU.Thirty healthy subjects matched for age and sex were compared as controls and all serum samples were analyzed for metabolites.Analysis of serum metabolism profiles of all subjects by ultra-high-performance liquid chromatography combined with quadrupole time-offlight mass spectrometry(UHPLC/Q-TOF-MS)feature.Metabolomics data were analyzed using a multivariate statistical approach,and Metabo-Analyst 4.0 analyzed the classical pathways associated with metabolites.Orthogonal partial least squaresdiscriminate analysis(OPLS-DA)was used to observe that EHT and EHT+HU metabolites were clearly separated,and 16 differential metabolites were identified as potential biomarkers.In addition,these metabolites are involved in the following classical metabolic pathways: linoleic acid metabolism,sphingolipid metabolism,steroid hormone biosynthesis,starch and sucrose metabolism,and purine metabolism.Serum metabolites in the healthy control group,EHT group and EHT+HU group were also clearly separated.Uric acid,L-lactic acid,and quinolinic acid continue to rise during the process from normal to EHT to EHT+HU.They are mainly involved in pyruvate metabolism,purine metabolism and niacin and nicotinamide metabolic pathways.Conclusion: Uric acid,L-lactic acid and quinolinic acid may play an important role in the progression from normal to EHT?EHT+HU.These identification of metabolites may help to understand the pathogenesis of EHT+HU and provide good prospects for preventing the combination of EHT and HU.