Effects Of Combined Signals Of Electrospun Nanofibers And Bioglass On Cell Behaviors And Its Application For Tissue Engineering

Structural signals or chemical signals of biomaterials can affect cell behaviors and tissue regeneration.However,the in vivo microenvironment is much more complicated than that in vitro.Cells and tissues always experience more than one type of stimulations during tissue regeneration,and there are interactions between different cells as well.Therefore,the effects of multiple stimulatory signals on cell behaviors,cell-cell communications and tissue regeneration are critical.As structural signals of electrospun nanofibers and chemical signals of bioglass(BG)have been reported to be able to impact cell behaviors and tissue regeneration,we studied the effects of combined signals of electrospun nanofibers and BG on cell behaviors and its application in tissue engineering.In this article,we investigated the effects of combined signals on human umbilical vein endothelial cells(HUVECs)and human dermal fibroblasts(HDFs).Results proved that BG could stimulate proliferation while electrospun nanofibers could influence adhesion and distribution of these two cells,and the combined signals showed stimulatory effects on angiogenic differentiation of HUVECs and matrix sythensis of HDFs.Then,we studied the effects of combined signals on interactions between co-cultured bone marrow stromal cells(HBMSCs)and HUVECs,as well as co-cultured HDFs and HUVECs.Results confirmed that combined signals could significantly stimulate interactions between co-cultured cells through both of paracrine effects and junctional communications,which resulted in promoted functional differentiation of co-cultured cells.Finally,in vivo results indicated that HDF-HUVEC co-culture constructs activated by combined signals could significantly promote wound contraction,enhance blood vessel formation and collagen I deposition in wound site,which eventually accelerated wound healing.Taken together,using combined structural signals and chemical signals of biomaterials to stimulate co-cultured cells may be a promising strategy for tissue engineering.