| Objective: Osteosarcoma(OS)is a serious primary skeletal malignancy along with high tendency to affect children and teenagers.Neoadjuvant chemotherapy,one of the important and feasible clinical therapeutic strategies for OS treatment,is drastically restricted due to the inadequate retention time,poor penetration ability,and severe side effects of current antitumor drug formulations.Hence,the calcium-mineralized doxorubicin-loaded polypeptide nonvehicle may have great potential to achieve high stability,selective intratumoral drug accumulation,and improved antitumor activity.Methods: The K7 murine osteosarcoma cell line and ten-week-old female BALB/C mice were utilized to examine the antitumor effect.The animal model was prepared by injecting the K7 cell to the right flank of the BALB/C mice.Then the drug loaded nanoparticle was synthesized and its efficacy was calculated by a series of examination including characterizations of nanoparticle,in vitro cell uptake and cytotoxicity,in vivo pharmacokinetics,biodistribution,antitumor efficacy,and security etc.Results: 1.Through the measurements of particle size with time can reveal that the particle size change of the Ca-mineralized group is smaller than that of the nonCa-mineralized group.2,Through the endocytosis experiment and tissue distribution experiment,it can be found that the Ca-mineralized drug carrier can achieve more aggregation in the tumor site.3,In contrast to free drug,the calcium-mineralized doxorubicin-loaded nanoparticle achieved better effect on tumor suppression and organ biocompatibility.The tumor inhibition rate of the experimental group(79.8±1.3%)was obviously higher than the free drug treated group 54.7±4.4%(P <0.01).Conclusion: 1,The Ca-mineralized experimental group achieved superior stability by calcium mineralization.2,This drug loaded system could perform enhanced tumor accumulation and stimulation-responded release in tumor.3,This drug loaded system could exhibit better tumor suppression and biocompatibility. |
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