The Detection And Clinical Significance Of Serum Neuron-specific Enolase And Myelin Basic Protein In Children With Cerebral Palsy

Objective: To investigate the changes and clinical significance of serum neuron-specific enolase(NSE)and myelin basic protein(MBP)in children with cerebral palsy.Methods: 46 cases of children with cerebral palsy is the observation group,all children with cerebral palsy were selected from child rehabilitation center in our hospital and their diagnosis basic on cerebral palsy diagnosis standard.In the same period,45 cases of sex and age matched healthy children is the control group,all the healthy children had physical examination in the Children's Health Departmen of our hospital.Children in the observation group were given conventional rehabilitation therapy such as exercise therapy,occupational therapy,acupuncture,massage,and so on.The serum levels of NSE and MBP in the control group at the time of physical examination and in the observation group before and after 3 months of routine rehabilitation were detected by enzyme linked immunosorbent assay(ELISA)and recorded respectively.The cerebral palsy Gross Motor Function Classification System(GMFCS)and the Gross Motor Function Measure 88 item(GMFM-88)were used to grade and evaluate the gross motor function before and after treatment in the observation group.Results:(1)The serum levels of NSE and MBP in the control group were respectively(6.77±2.08 ?g/L)and(0.38±0.10 ?g/L);the serum levels of NSE and MBP in the observation group before treatment were respectively(16.26±2.94 ?g/L)and(1.21±0.41 ?g/L),and the serum levels of NSE and MBP in the observation group after 3 months of treatment were respectively(13.56±2.29?g/L)and(0.85±0.33?g/L);the GMFM-88 scores of the observation group before and after treatment were respectively(132.33±81.60)and(147.07±83.13).(2)There was no significant correlation between NSE and MBP levels and age in healthy children of the control group(P>0.05),and there was a negative correlation between NSE and MBP levels and age before treatment in the observation group(P<0.05).(3)There were significant differences in the levels of NSE and MBP between the control group and the observation group(P<0.05).(4)There was a positive correlation between NSE and MBP levels of the observation group and Gross Motor Function Classification System(GMFCS)in children with cerebral palsy,and a negative correlation with GMFM-88 score(P<0.05).(5)The differences of NSE,MBP and GMFM-88 scores in the observation group before and after treatment were statistically significant(P<0.05).Conclusion:(1)Serum NSE and MBP levels in healthy children do not change with age.(2)Serum NSE and MBP levels in children with cerebral palsy were significantly higher than those in healthy children,and decreased with age,and correlated with the degree of cerebral palsy and gross motor function.(3)NSE and MBP can be used as a specific objective indicators to evaluate the brain injury in children with cerebral palsy.(4)NSE and MBP can be used as biochemical indicators to help evaluate the therapeutic effect of cerebral palsy.