A Study Of Anti-cancer Effect Of Astragalus Polysaccharides On Colorectal Cancer And Its Correlation With Lymphangiogenesis

Objective: To study the effects of astragalus polysaccharides on the proliferation of human colon cancer LOVO cells and normal human lymphatic endothelial HLEC cells,and the expression of lymphatic endothelial growth factor VEGF-C protein and mRNA.To explore the relationship between the anti-cancer effect of Astragalus polysaccharides on colorectal cancer and lymphangiogenesis.Methods: Commercial human colon cancer LOVO cell line and normal human lymphatic endothelial HLEC cell line were treated with different concentrations of astragalus polysaccharides(0.5 mg/ml,1 mg/ml,1.5 mg/ml,2 mg/ml).After 24 h,48h,72 h and 96 h,CCK-8 method was used to detect the activity and proliferation of human colon cancer LOVO cells and normal human lymphatic endothelial HLEC cells.The effects of Astragalus polysaccharides on two kinds of cells and their optimal concentration and optimal action time were studied.The effect of Astragalus polysaccharide on the expression of VEGF-C protein in human colon cancer LOVO cells and normal human lymphatic endothelial HLEC cells was detected by Western Blot.Reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the effect of Astragalus polysaccharide on the expression of VEGF-C mRNA in human colon cancer LOVO cells and normal human lymphatic endothelial HLEC cells.SPASS 21.0 software was used for data analysis with ?=0.05 as the test level.Results:After treatment with different concentrations of Astragalus polysaccharides(0.5mg/ml,1mg/ml,1.5mg/ml,2mg/ml)for 24 h,48h,72 h and 96 h,the cell viability and proliferation levels of human colon cancer LOVO cells decreased significantly.The difference in the control group was statistically significant(P<0.05),and it was concentration and time dependent.However,the effect on the normal human lymphatic endothelial HLEC cells was opposite(P<0.05).The optimal concentration is 1.5 mg/ml,and the time is 48 h.The expression of VEGF-C protein in human colon cancer LOVO cells treated with different concentrations of Astragalus polysaccharides was significantly lower than that in the control group(P<0.05),and it was dose dependent.For normal human lymphatic endothelial HLEC cells,the expression of VEGF-C was up-regulated when the concentration of Astragaluspolysaccharide was 2 mg/ml,and the difference was statistically significant(P<0.05).When the concentration of Astragalus polysaccharide was less than 2mg/ml,the difference was not significant(P>0.05).The expression of VEGF-C mRNA in human colon cancer LOVO cells was decreased after treatment with Astragalus polysaccharides(P<0.05),which was time and dose dependent.The normal human lymphatic endothelial HLEC cells treated with Astragalus polysaccharides had a concentration of Astragalus polysaccharide less than 1.5 mg/ml.When the time was less than 8 h,the expression of VEGF-C mRNA was increased,but there was no significant difference(P>0.05).Conclusion: Astragalus polysaccharide can inhibit the cell activity and proliferation of human colon cancer LOVO cells in a dose and time dependent manner,and can down-regulate the expression of VEGF-C.A certain concentration of Astragalus polysaccharide can enhance the activity of normal human lymphatic endothelial HLEC cells.The anti-cancer effect of Astragalus polysaccharides on colorectal cancer may be related to the down-regulation of VEGF-C expression in tumor cells and inhibition of lymphangiogenesis.