Research On Key Technologies Of Cell Marker System Based On Energy Metabolism Time Domain Characteristic Method

Parallel optical coherence tomography(POCT)is a new medical imaging technology based on the principle of weak interference of broad-spectrum weak low-coherence light source.It is mainly used to obtain high resolution,deep penetration and high contrast biological tissue images.It has many advantages such as non-contact,non-destructive,high resolution and real-time imaging.It has been widely used in outpatient pre-research,cardiovascular imaging,biological laboratory tissue detection and other fields.At present,POCT image extraction algorithms mainly use phase shifting algorithms(such as second-order phase shifting,fourth-order phase shifting,etc.).The Boccara team of Langevin Institute in France proposed to use time-domain image signals to dynamically solve POCT images to achieve bio-tissue feature image extraction,which is called "dynamic POCT" image.This paper focuses on the adjustment of dynamic POCT image systems,image resolution algorithms and noise suppression techniques.This paper describes the principle and research trends of POCT system.Combining with the requirements of biological tissue imaging,a POCT imaging system with Linnik interference structure as the core is built.The system consists of light source arm(including incoherent LED light source,collimation beam expanding structure),sampling arm(including microscopic objective and sample chamber),interference arm and detection arm(including displacement table,area array CCD detector)and control and calculation computer.Form.The interference length of the light source in POCT system is only micron level.The micron level alignment error of the reference arm and the sampling arm and the tilt of the milliarc refractive elements will lead to the loss of interference fringes and the system failure.In this paper,the iterative adjustment method of the defocusing amount corrected by the light source and the camera is creatively adopted.The focal depth of the optical element above 10 micron and the depth of the micron interference pattern are iterated.By adjusting,high precision element positioning is realized and high contrast interference fringes are obtained.According to the characteristics of POCT imaging,the noise sources and characteristics of POCT system are analyzed in detail,and a dynamic POCT image demodulation scheme combining denoising method with standard deviation calculation is proposed.The effectiveness of median denoising,mean denoising and wavelet threshold denoising algorithms is analyzed by using MATLAB simulation.The results of numerical simulation show that compared with traditional denoising methods such as median denoising and mean denoising,the threshold denoising method based on wavelet transform combined with standard deviation calculation has higher signal-to-noise ratio,which lays a good numerical foundation for high-quality dynamic POCT image extraction.