The Research Of Relationships Between MTHFR,MTRR Gene Polymorphism And Plasma Homocysteine,Folate In H-type Hypertension Patients

Objectives:To investigate the relationship between MTHFR C677T,MTHFR A1298C and MTRR A66G genetic variants with respect to both plasma homocysteine concentration and folate in H-type hypertension,in order to provide the theoretical basis for personalized treatment.Methods:This study enrolled 170 hypertensive patients in the Kunming top three hospital from June 2017 to June 2018,plasma homocysteine and folate were measured by chemiluminescence microparticlelmmuno assay,according to the Hey levels,170 residents were divided into two groups:an H-type hypertension group(n=85),a non H-type hypertension group(n=85).Known genotypes of Methylenetetra-hydrofolate reductase(MTHFR)C677T and A1298C,and Methionine synthase reductase(MTRR)A66G were detected by PCR methods.These datas were analysised by SPSS 20.0 software.Results:1.Amomg the 170 hypertension peoples,H-type hypertension group the frequencies of the 677T allele were significantly higher than non H-type hypertension group(55.3%and 30.6%,respectively)for MTHFR C677T(P<0.05).With the H-type hypertension group,the frequencies of MTHFR 677CC,CT,TT genotypes were 22.4%,44.7%and 32.9%,respectively.And non H-type hypertension the frequencies of MTHFR 677CC,CT,TT genotypes were 47.1%,44.7%and 8.2%,respectively.There was a statistical difference between the two groups(P<0.05).No significantly differenceswere foud in the MTHFR A1298C and MTRR A66G allele and genotype between the two groups(P>0.05).2.The MTHFR C677T genotype mutation was an independent genetic risk factor for H.type hypertension(P<0.05),we found that a significantly increased risk of H-type hypertension was associated with the variant genotypes of 677CT and TT genotype(OR=8.421,95%CI:3.123-22.710,P<0.05;OR=4.000,95%CI:1.559-10.265,P<0.05).3.There was statistical significance between MTHFR C677T gene polymorphism and plasma Hey and folate concentrations(P<0.05),compared to subjects with CT and CC genotype,homocysteine concentrations remained higher in subjects with TT genotype(r=0.487,P<0.05).Furthermore,subjects with TT genotype showed a lower folate than did subjects with CT and CC genotype(r=-0.424,P<0.05).There was no statistical significance between MTHFR A1298C,MTRR A66G gene polymorphism and plasma homocysteine or folate concentrations in H-type hypertension group(P>0.05).4.There were significant differences in plasma homocysteine levels and hypertension grade of in H-type hypertension patients(P<0.05).Hcy levels were associated with an increased risk of incident hypertension(r=0.622,P<0.05).5.Correlation analysis between plasma homocysteine and folate levels in H-type hypertension patients showed that folate was negatively correlated with homocysteine(r=-0.522,P<0.05).Conclusions:1.MTHFR C677T gene mutation was an genetic predisposition factor for H-type hypertension.2.The mutation of MTHFR C677T gene caused the increase of plasma homocysteine and decreased of plasma folate level,no association were found between MTHFR A1298C,MTRR A66G genetic variants and plasma homocysteine and folate levels.Low folate status and MTHFR C677T gene polymorphisms may have a synergistic effect increased the incidence of hyperhomocysteinemia in H-type hypentension patients.3.There was a positive correlation between plasma homocysteine level and hypertension grade,higher levels of homocysteine were associated with an increased of hypertension,it was necessary to intervene the plasma homocysteine level while controlling blood pressure for H-type hypentension patients.4.There was a significant negative correlation between plasma folate and homocysteine level.Folic acid was a safe and effective supplement that may reduce plasma homocysteine level effectively for H-type hypentension patients.The H-type hypentension patients of homozygous mutation of MTHFR C677T gene generally had a high level of homocysteine and a low level of folic acid.Therefore,we recommend that the H-type hypentension patients of homozygous mutation of MTHFR C677T gene should be appropriately increased the dosage of folic acid supplements.The results suggest that folic acid treatment may be beneficial to reduce homocystein in hypertensive patients with high homocystein.