The Study Of The C677T Polymorphism Of MTHFR Gene In The Tibetan And Han Patients With H-type Hypertension In Qinghai Province

Objective: To study whether there are ethnic differences in the distribution of methylene tetrahydrofolate reductase(Methylene Tetrahydrofolate Reductase,MTHFR)C677T genotypes and alleles frequency between Tibetan and Han patients with H-type hypertension in Qinghai Province,and determine whether there are differences in the distribution of MTHFR C677 T genotypes and alleles frequency between H-type and non H-type hypertension patients.To discuss the relationship between C677 T polymorphism of MTHFR gene and H-type hypertension and homocysteine(Homocysteine,Hcy)level in the Tibetan and Han patients with H-type hypertension in Qinghai Province.Methods:From February in 2018 to September in 2019,46 cases of Tibetan patients with H-type hypertension,27 cases of Tibetan patients with non H-type hypertension,49 cases of Han patients with H-type hypertension and 39 cases of Han patients with non H-type hypertension were randomly selected to be hospitalized in the department of cardiology of the affiliated hospital of Qinghai university.Hcy,biochemical and carotid ultrasonography were performed in all patients,and C677 T polymorphism of MTHFR gene was detected.Results:(1)Compare the differences in the general clinical data of the subjects in each group.Among them,analysis results showed:1)The Hcy and creatinine(Cr)in the Tibetan and Han groups with H-type hypertension were significantly higher than that in the non H-type hypertension group(p<0.01).2)The uric acid(UA)in the Han groups with H-type hypertension was significantly higher than that in the non H-type hypertension group(p<0.001).(2)There were significant differences in the MTHFR C677 T genotypes and alleles frequency among the four groups(p<0.05).Among them,the frequency of TT genotype and T allele in both Tibetan and Han groups with H-type hypertension was higher than that in non H-type hypertension group(p<0.05),and the frequency of TT genotype and T allele in the Han group with H-type hypertension was higher than that in the Tibetan group(p<0.05).There were no significant differences in CC genotype,CT genotype and C allele frequency between four groups(p > 0.05).(3)The Hcy level of TT genotype in the Tibetan and Han groups with H-type hypertension was higher than that of CC and CT genotypes,and the differences were statistically significant(p<0.01),but there was no significant difference in Hcy level between CC genotype and CT genotype(p>0.05).(4)Results of binary logistic regression analysis: TT genotype is a risk factor of H-type hypertension in Tibetan and Han nationality in Qinghai Province(H-Type hypertension of Han nationality: p=0.027,OR 7.494,95%CI 1.264-44.437;H-Type hypertension of Tibetan nationality: p=0.010,OR 10.636,95%CI 1.770-63.927).Conclusion:(1)The frequency of TT genotype and T allele in both Tibetan and Han groups with H-type hypertension was higher than that in non H-type hypertension group in Qinghai Province.The frequency of TT genotype and T allele in the Han group with H-type hypertension was higher than that in the Tibetan group.(2)The C677 T polymorphism of MTHFR gene is related to the level of plasma Hcy in the Tibetan and Han patients with H-type hypertension in Qinghai Province,and the increase of Hcy level caused by TT genotype was significant.TT genotype is a risk factor of H-type hypertension in Tibetan and Han nationality in Qinghai Province(3)The level of Cr in Tibetan groups with H-type hypertension was higher than that in Tibetan groups with non H-type hypertension group,and UA and Cr in Han groups with H-type hypertension group were higher than that in Han groups with non H-type hypertension group.