Research On Genetic Susceptibility Genes CD36 And EVL,about Familial Aggregation Of Stroke

ObjectiveTo Study on the differences in the expression of CD36 and EVL gene between different types of stroke patients and normal controls,and explore whether CD36 and EVL gene are the susceptibility genes of familial aggregation of stroke.MethodOn clinic,we collect the general clinical data and venous blood specimens of a total of 73 cases of the research object,including: 21 cases of familial aggregation of cerebral thrombosis,18 cases of sporadic cerebral thrombosis,4 cases of familial aggregation of cerebral hemorrhage,16 cases of sporadic cerebral hemorrhage and 14 cases of normal control group.RNA extracted from whole blood,reverse transcribed into cDNA,using real-time quantitative PCR technology,illuminate the relative expression of target gene CD36 and EVL of each group of sample,finally by the statistical analysis whether the difference of target gene expression in five groups have statistical significance.Results1.Familial aggregation of cerebral thrombosis group,sporadic cerebral thrombosis group,familial aggregation of cerebral hemorrhage group,sporadic cerebral hemorrhage group compared with normal control group,the differences in age,smoking history,drinking history,history of hypertension,history of diabetes,systolic blood pressure,diastolic blood pressure,fasting blood glucose,blood lipids had no statistical significance(P>0.05).2.The expression of CD36 gene in familial aggregation of cerebral thrombosis group,sporadic cerebral thrombosis group were significantly increased compared with the normal control group,and the difference was statistically significant(P<0.05).3.The expression of EVL gene in familial aggregation of cerebral thrombosis group,familial aggregation of cerebral hemorrhage group were significantly increased compared with the normal control group,and the difference was statistically significant(p<0.05).Conclusions1.CD36 gene may be a susceptibility gene of familial aggregation of cerebral thrombosis,and the increase of its expression may increase the susceptibility of cerebral thrombosis.2.EVL gene may be a susceptibility gene of familial aggregation of cerebral thrombosis and familial aggregation of cerebral hemorrhage,the increase of its expression may increase the susceptibility of stroke.