Bioinformatics Analysis Of Differentially Expressed Genes And Key Pathways In Castration Resistant Prostate Cancer

ObjectAs the most common malignant tumor in the male genitourinary system,prostate cancer is one of the major diseases that threaten the health of men throughout the world.Although the incidence of prostate cancer in China is still at a relatively low level compared with Western countries,in recent years,with the changes in the way of life of Chinese people and the increase of healthy physical examination consciousness,the incidence rate of prostate cancer has increased significantly.In recent years,with the advancement of science and technology and the rapid development of molecular biology,people have gained more understanding of the occurrence and development process of prostate cancer.However,the occurrence and development process of prostate cancer is very complicated.It has the characteristics of multi-path changes,multiple gene recombination and so on.People still do not fully understand the specific mechanisms.Current research has found that most prostate cancer patients have a significant effect after the first treatment with androgen deprivation.Therefore,prostate cancer cells have biological properties that depend on the growth of androgens,but most prostate cancer patients eventually relapse,many develop from androgen-dependent prostate cancer to androgen-independent prostate cancer,and are characterized by high levels of deterioration and widespread metastasis,the conventional androgen deprivation therapy loses its effect at last.This is also the main reason for the low disease-free survival and high mortality rate of castration-resistant prostate cancer patients.At present,there are many reports on the mechanism of prostate cancer.However,there is still no consensus on the mechanism of formation of castration-resistant prostate cancer,There is no unified standard for the choice of treatment.Therefore,the search for a mechanism for the transformation of prostate cancer castration needs more efforts.This study uses bioinformatics methods to explore the molecular mechanism of endocrine therapy resistance in castration-resistant prostate cancer(CRPC),Search for potential therapeutic targets for castrated resistance to prostate cancer.And provide a new direction for clinical diagnosis and treatment of prostate cancer patients.MethodThe Prostate cancer related gene microarray data were extracted from NCBI's Gene Expression Omnibus(GEO)database,use GEO2 R online analysis tool to screen out the different expressed genes of castration-resistant prostate cancer and hormone-sensitive prostate cancer,and use DAVID online analysis software for functional enrichment analysis of related genes.Subsequently,the protein-protein interaction(PPI)network was constructed using STRING and Cytoscape software.ResultThere were found 270 DEGs,of which 141 genes were upregulated and 129 genes were downregulated(logFc>1.5,P<0.01).The identified significant hub DEGs included TOP2A?CDK1?BIRC5?BUB1?AURKA?FOXM1?DCN?EZH2?TYMS?ASPM.Finally,the literature mining found that these genes may be related to the resistance and metastasis of prostate cancer.ConclusionUsing bioinformatics methods to analyze DEGs in CRPC,can effectively discover the interaction information of these differentially expressed genes,and may develop new targets for clinical treatments.