| Objective: This experiment is to study the effect of Sophora flavescens Ait on the growth,acid production,sugar production and adhesion virulence factors of planktonic and biofilmcausing bacteria,and to explore its anti-caries mechanism.Methods: The minimum inhibitory concentration of the extract of Sophora flavescens Ait was determined by double gradient dilution method,0.5g/L chlorhexidine was used as the positive control,and the drug-free group was used as the negative control group;the bacterial adhesion was determined by ultraviolet spectrophotometer;Biofilm inhibition concentration and biofilm removal concentration were determined by membrane crystal violet staining;bacterial acid production and synthetic water insoluble extracellular polysaccharide were determined by ?pH method and phenol-sulfuric acid method,respectively.Results: The minimum inhibitory concentration of Sophora flavescens Ait on the main cariogenic bacteria was 4g/L;at this concentration,it could significantly inhibit the adhesion of the main cariogenic bacteria,and the inhibition rate is between 60.68% to 85.1%;When the concentration of Sophora flavescens Ait at 2g/L,it significantly inhibited the cariogenic bacteria's ability to produce acid and synthesize water-insoluble extracellular polysaccharides.The minimum biofilm formation inhibitory concentration of Sophora flavescens on 80% of the main cariogenic bacteria was 4g/L;The acid production and synthetic water of the Single bacterial biofilm of the main cariogenic bacteria by Sophora flavescens on the minimum biofilm removal concentration of 50% The inhibitory rates of insoluble extracellular polysaccharides were 67.5% to 94.1% and 42.3% to 60.0%,respectively.Conclusion: Sophora flavescens Ait could inhibit the growth,adhesion,acid production and sugar production of the major caries-causing bacteria in the planktonic and biofilm states,and it has a certain effect on preventing dental caries. |
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