The Role Of Kallikrein-kallikrein System And Inflammatory Factor In ED Rats

Objective: To study the changes in the expression of kallikrein-kallikrein system and inflammatory factors in the ED rat model of the penis,and to explore the possible mechanism and biological significance in the development of ED.Methods: 120 healthy mature male SD rats were enrolled in this study,20 of them were taken randomly as the control group(N),the remainder 100 were in the model group.The ED rat model was made with compound factors,and the ED model rats were determined by mating and APO erectile tests.The rats were randomly divided into ED model group(ED group)and drug treatment group(Y group).At the end of the experiment,penis tissue was taken and total RNA and total protein were extracted,paraffin-embedded and sectioned,and subjected to reverse transcription-polymerase chain reaction(RT-PCR),western-blot and immunohistochemistry(IHC),respectively detection of T-kininogen,kallikrein 1 and other kallikrein-kinin system and TNF-?,TGF-?1 Such as inflammatory factors and endothelial cell specific markers CD31 and other key indicators.Results:(1)Model result: the ED rat model was successfully replicated.(2)Changes of endothelial cell specific markers CD31 expression level :The results of RT-PCR display CD31 in ED group were significantly decreased with the normal group,respectively after drug intervention,ED were significantly increased(P<0.05).The results of immunohistochemistry display the CD31 in rat penile tissue of urethra,penis cavernous sinus endothelial and vascular endothelial were expressed,compared with the normal group,ED group of CD31 expression decreased significantly after tablet after intervention significantly increased.(3)Changes of Kallikrein-kinin System expression level :The results of RT-PCR display kallikrein 1 in ED group were significantly decreased with the normal group,respectively after drug intervention,ED were significantly increased(P<0.05);T-kininogen1 in the ED group was significantly increased compared with normal group,respectively after drug intervention were significantly decreased with syndrome and disease model group(P<0.05).Westren-blot experiment display the expression of kallikrein 1,T-kininogen 1 was consistent with RT-PCR.Kallikrein 1 in ED groups were significantly decrease with the normal group.After drug intervention,all ED groups increased significantly(P<0.05);T-kininogen 1 in ED group was significantly increased compared with the normal group,after drug intervention,all were significantly decreased in ED group(P<0.05).The results of immunohistochemistry display the kallikrein 1,T-kininogen 1 in rat penile tissue of urethra,penis cavernous sinus endothelial and vascular endothelial were expressed,compared with the normal group,ED group of kallikrein 1 expression decreased significantly after tablet after intervention significantly increased(P<0.05);T-kininogen 1 expression was significantly increased compared with the normal group,significantly decreased after drug intervention(P<0.05).(4)Changes in the expression of inflammatory factors :The results of RT-PCR display TNF-? and TGF-?1 in the ED group was significantly increased compared with normal group,respectively after drug intervention were significantly decreased with syndrome and disease model group(P<0.05).Westren-blot experiment display the expression of TNF-?,TGF-?1 in ED group was significantly increased compared with the normal group,after drug intervention,all were significantly decreased in ED group(P<0.05).Conclusions(1)The ED rat model was successfully produced,and the Yimusake tablet could significantly improve penile erection function of ED rats.(2)Kallikrein 1,T-kininogen 1 participates in the development of ED,and damages the endothelial function of the penile cortex due to ED,and the mechanism of Yimusake tablets in the treatment of ED and the regulation of kallikrein 1,T-kininogen 1 is involved.(3)Decrease of vascular endothelium marker CD31 indicates the presence of vascular endothelial dysfunction in the development of ED,while decreased kallikrein1 and increased T-kininogen1 lead to the expression of TNF-? and TGF-?1.Elevation may be the direct cause of impaired endothelial function in cavernosal,and Yimusake tablets can significantly improve cavernosal endothelial function.