Analysis Of The Mutation Of Melanoma Susceptibility Gene

Objective: To identify and locate the mutations of BRAF,NRAS,c-KIT and hTERT gene promoter in melanoma,and analyze the correlation between the mutations and the clinicopathological characteristics of melanoma.Methods: The 48 paraffin tissue samples and 40 blood samples of melanoma were collected from dermatology and pathology department of Xinjiang Uygur Autonomous Region People's Hospital from January 2010 to October 2019.The mutation of BRAF,NRAS,c-KIT gene and hTERT gene promoter region in melanoma tissue and blood samples were detected by PCR and Sanger sequencing.Results: In this study,11 of 48 melanoma tissue samples showed BRAF gene mutation,with a mutation rate of 23.1%(11 / 48);5 NRAS gene mutation,with a mutation rate of 10.4%(5/48);6 c-KIT gene mutation,with a mutation rate of 12.5%(6/48);7hTERT gene promoter region mutation,with a mutation rate of 14.6%(7/48);BRAF gene mutation in non acral melanoma patients The rate was higher(P < 0.05);the more likely there was gene mutation in high-risk cases with lymph node metastasis;there was no significant difference in the mutations of BRAF,NRAS,c-KITt gene and hTERT gene promoter region in patients with melanoma of different gender and ethnicity(P>0.05).Conclusion: In this study,the incidence of melanoma was mostly seen in the middle-aged and old people,with little gender and ethnic differences,mainly acromegaly.The mutations of BRAF gene,NRAS gene,c-kit gene and hTERT gene promoter in melanoma were not significantly correlated with gender and nationality.There are different gene mutations in melanoma patients at the same time.BRAF gene mutation is related to hTERT gene promoter mutation.It can provide theoretical basis for the choice of targeted therapy.In high-risk cases with lymph node metastasis,there is a high possibility of gene mutation.In late stage and metastasis patients,molecular targeted drugs are more suitable.