| Objective: To study the protective effect of Shenmai Fang on the heart of type II diabetic rats,the protective effect of Shenmai injection on HUVECs endothelial cells,and to study the specific protective mechanism.Methods: 1.Protective effect of Shenmaifang on the heart of type II diabetic rats:In this experiment,SD male rats were combined with intraperitoneal injection of streptozotocin and fed with high-sugar and high-fat diet to construct a diabetic cardiomyopathy model.By observing the general vital signs,weight,and blood glucose of rats,the rat’s model formation was judged.A rat model of type II diabetes was established,and high-sugar and high-fat feed was continued to build a model of diabetic cardiomyopathy.In order to determine the efficacy of the drug,the experiment was divided into five groups,namely the normal group,the model group,the positive control group,the Shenmai low-dose group,and the Shenmai high-dose group.Detect fasting blood glucose in rats,determine the hypoglycemic effect of the drug,calculate the cardiac index of rats,make cardiac pathological sections for observation,detect the ultrastructure of myocardium and autophagosomes,and determine the morphological changes of myocardium in rats.Heart damage was judged by serum creatine kinase(CK)andα-hydroxybutyrate dehydrogenase(HBDH)contents.Based on the levels of triglyceride(TG),aspartate aminotransferase(AST),and aspartate / alanine aminotransferase(AST/ALT)in the blood,determine whether the drug is reducing lipids and preventing fatty liver Aspect effect.2.Protective effect of Shenmai Fang on HUVECs endothelial cell cobalt chloride injury: establish a HUVECs endothelial cell cobalt chloride injury model,observe the cell growth status with an inverted fluorescence microscope,and take Shenmai injection group as a positive control drug The experiment was performed with the prescription drug-containing serum group,and the experiment was divided into 5 groups: blank group,normal group,model group,Shenmai injection group,and Shenmai drug-containing serum group.An inverted fluorescence microscope was used to observe the cell growth status,the cell survival rate was measured by the CCK8 method,the apoptosis was observed by Annexin V-PI doublestaining,and the mitochondrial membrane potential was detected by JC-1.Results: 1.Protective effect of Shenmaifang on the heart of type II diabetic rats:Type II diabetic rats have obvious three-pronged phenomenon,dull coat,and poor mental state.Metformin hydrochloride group,Shenmaifang low-dose group,and Shenmaifang high-dose group can significantly improve the above phenomenon,and the Shenmaifang group is more effective than metformin The improvement was obvious in the group and the lower dose group in the Shenmaifang high-dose group;The body weight of type II diabetic rats decreased significantly(P<0.001).The metformin hydrochloride group and Shenmaifang high-dose group could increase the weight of diabetic rats.The Shenmaifang low-dose group showed weight gain in diabetic rats two weeks before administration.;Fasting blood glucose and fasting blood glucose in the tail vein of type II diabetic rats increased significantly(P<0.001).Metformin hydrochloride group,Shenmaifang low-dose group,and Shenmaifang high-dose group did not significantly improve blood glucose in diabetic rats;II Serum creatine kinase(CK)has an increasing trend in type II diabetic rats.Serum creatine kinase(CK)in the metformin hydrochloride group,the Shenmaifang low-dose group,and the Shenmaifang high-dose group has a decreasing tendency,and the Shenmai formula is low.The dose group showed a significant downward trend;the serum α-hydroxybutyrate dehydrogenase(HBDH)in type II diabetic rats showed an upward trend.The enzyme(HBDH)showed a downward trend,and the Shenmai high-dose group had a significant difference(P<0.05).The metformin hydrochloride group did not decrease the rat serum α-hydroxybutyrate dehydrogenase(HBDH);type II diabetic rats Cardiac Index Rise High,metformin hydrochloride group,Shenmaifang high-dose group showed a decrease in cardiac index,while Shenmaifang low-dose group did not show a decrease in cardiac index;serum type II triglyceride(TG)increased in rats with type II diabetes.Significance(P<0.05),the serum triglyceride(TG)of rats in the metformin hydrochloride group,the Shenmaifang low-dose group,and the Shenmaifang high-dose group has a downward trend.The low-dose group has a significant decrease compared with the high-dose group of Shenmai Fang;the serum aspartate aminotransferase(AST)of type II diabetic rats has an upward trend.Themetformin hydrochloride group,the low-dose Shenmai group,and the high-dose Shenmai Fang have a rising trend.The serum AST of the rats in the group had a downward trend.The Shenmaifang group had a significant decline compared with the metformin hydrochloride group,and the Shenmaifang low-and high-dose groups had a significant decline compared with the Shenmaifang low-dose group.The serum aspartic acid / alanine in type II diabetic rats Acid aminotransferase(AST/ALT)has an increasing trend.The serum AST/ALT of the metformin hydrochloride group,the Shenmaifang low-dose group,and the Shenmaifang high-dose group has a decreasing trend.The Shenmaifang group has a lower decline than the metformin hydrochloride group.Obviously,the Shenmaifang low-dose and high-dose group decreased significantly compared with the Shenmaifang low-dose group,and the Shenmaifang low-and high-dose had statistical significance(P<0.05);pathological observation of Shenmaifang could improve myocardial hypertrophy,necrosis,and fibrosis.,Has a protective effect on the heart;Shenmai formula can reduce the rate of apoptosis.2.Shenmai Fang’s protective effect on HUVECs endothelial cell cobalt chloride injury: Cobalt chloride injury can significantly reduce HUVECs endothelial cell survival rate(P <0.001),and Shenmai two groups can improve HUVECs endothelial cell survival rate(P<0.05).By fluorescence staining,it was found that compared with the normal group,the apoptosis of the model group was severe,the apoptosis of each administration group was reduced,the mitochondrial membrane potential of the model group cells was reduced,and the state of the Shenmai group was alleviated.Conclusion: 1.Shenmai Fang can improve the body weight,blood lipid,cardiac index,myocardial enzyme spectrum and liver function of diabetic rats,but it has no significant effect on blood glucose.Pathological sections showed that Shenmai Fang could effectively improve the degree of myocardial hypertrophy and fibrosis in diabetic rats,and had a protective effect on the heart.2.Shenmai Fang can promote cell proliferation,and has certain protective ability against hypoxic-damaged cells,which can reduce the apoptosis level of cells under hypoxic conditions,and the Shenmai injection group is more effective in effect. |
