The Expression Of HOXA7 In Glioma And The Effect On Proliferation Of Glioma Cells By Proliferation Down-Regulation

BackgroundGlioma is a malignant tumor of the central nervous system derived from neurons and glial cells.It has the characteristics of high morbidity,high mortality,and high metastasis rate.Although the medical level is constantly improving,the tumor-free survival time of glioma patients has not improved significantly.Therefore,the urgent problem for humans is to find new targets to treat glioma.Studies have found that HOXA7 is highly expressed in a variety of malignant tumor cells.However,the relationship between HOXA7 and glioma is unknown.Therefore,the purpose of this study is to investigate the expression of HOXA7 in glioma and the effect on glioma cell proliferation.PurposeThis study intends to detect the expression of HOXA7 through human glioma tissues,and use HOXA7 siRNA to transfect glioma cells to further clarify the effect of HOXA7 on glioma cell proliferation and apoptosis,with a view to providing new clinical diagnosis and treatment of glioma Target.MethodFirst,the CGGA and Oncomine databases were used to analyze the expression of HOXA7 in glioma and its relationship with the glioma WHO grade and prognosis.Second,real-time PCR experiments were used to detect the expression of HOXA7 mRNA in glioma tissue and normal control brain tissue.Then,HOXA7 siRNA was transfected and the expression of HOXA7 mRNA in transfected cells was detected by real time PCR.Finally,CCK8 experiment was used to detect the expression of proliferation and changes in cell cycle and apoptotic rate of U251 that transfection with HOXA7 siRNA.Results1.The expression of HOXA7 in the glioma database and its relationship with WHO grade and prognosis of gliomaWe first performed data analysis by using the Chinese Glioma Genome Atlas(CGGA)and the cancer gene chip oncomine database.The higher the WHO glioma level,the higher the HOXA7 expression level.HOXA7 expression is closely related to glioma molecular subtypes,corresponding drugs,and sensitivity to chemotherapy.And the survival time of glioma patients with high expression of HOXA7 was significantly shortened.2.The expression of HOXA7 in tumor tissue of glioma patientsWe selected human glioma tissue samples with different grade in our hospital and normal brain tissue samples as control,and detected the expression of HOXA7 in human glioma tissues by using real time PCR experiments.We found that the expression of HOXA7 in brain glioma tissues of high-grade was significantly higher than low-grade human glioma tissues,and there is a positive correlation between the levels of grade in glioma tissues.As the malignancy of gliomas increased,the expression of HOXA7 increased significantly.3.The effect of HOXA7 siRNA on U251 human glioma cell proliferation.Subsequently,through CCK8 experiments,we found that compared with blank and nonsense sequence scrambled siRNA groups,the cell proliferation ability of glioma U251 cells in the HOXA7 siRNA group was significantly inhibited.4.Changes of U251 cell cycle and apoptosis after transfection with HOXA7 siRNAFlow cytometry showed that the cells were arrested in the G0/G1 phase after knocking down HOXA7,and the apoptosis results showed that the apoptosis rate in the HOXA7 siRNA group was significantly higher than that in the blank group and the scrambled siRNA group with nonsense sequences.Conclusions1.HOXA7 is highly expressed in glioma tissues,and the expression of HOXA7 is positively correlated with glioma grade and negatively correlated with survival;2.HOXA7 siRNA significantly inhibits the proliferation of U251 human glioma cells;3.HOXA7 siRNA may block the cell cycle in G0 / G1 phase and increase the apoptosis rate,and lead to the inhibition of U251 human glioma cells proliferation.