Expression And Significance Of ABCG2 In U251 And U251 Stem Cells

Objective This study was to investigate the expression and significance of ABCG2((the G2 member of ATP- binding cassette family) in U251and its stem cell.Methods The U251 were cultured in serum-free medium with EGF, bFGF,LIF and B27.The tumor spheres formed and cultured continuously in the serum-free medium through the monoclonal formation experiment. The immunofluorescence staining was employed to identificate the brain cancer stem cell. The expression of ABCG2 was detected by confocal microscopy and western blot.Results The stem cells from malignant glioma cell line U251 have been successfully isolated. The cells expressed CD133 and nestin. The ABCG2 protein positive stain was located in the cytomember with confocal microscopy method, Western blotting show ABCG2 was expressed in U251 cells and in its stem cells and the expression level of U251 stem cells significantly higher than U251 cells. P<0.05.Conclusion ABCG2 expressed in U251 cells and in glioma stem cells, but the expression level in the latter was higher.