The Effect Of B7-h3 Silencing On The Radiosensitivity Of Human Nasopharyngeal Carcinoma 5-8f Cells

Aim: studied the effect of B7-H3 expression silencing on the radiosensitivity of nasopharyngeal carcinoma 5-8F cells and possible molecular mechanism.Methods: we established B7-H3 shRNA stably expressing nasopharyngeal carcinoma cell line shRNA-B7-H3 and nonrelevant sequence-containing vector stably expressing nasopharyngeal carcinoma cell line pLKO-5-8F by lentivirus mediated gene expression silencing technology.Fixed source skin distance(SSD)technology was adopted to radiate wild type group 190-5-8F cells,control group pLKO-5-8F cells and B7-H3 expression silencing group shRNA-B7-H3 cells.By analyzing colony formation efficiency,proliferation efficiency and morphological changes of apoptosis of these cells after radiotherapy.Results: Silencing B7-H3 expression can significantly inhibit the colony formation and cell proliferation and promote the apoptosis of nasopharyngeal carcinoma 5-8F cells after radiotherapy.Caspase-3 expression is upregulated and Bcl-2 expression is downregulated in B7-H3 silencing 5-8F cells after radiotherapy.Conclusion: silencing B7-H3 expression can promote cell apoptosis signaling pathway possibly by upregulating Caspase-3 and downregulating Bcl-2 expression level,and increase the radiosensitivity of nasopharyngeal carcinoma 5-8F cells.