Association Study Of EP300 Rs20551 Polymorphism And Phlegm-blood Stasis Syndrome Of Ischemic Stroke And Coronary Heart Disease

Objective:This study explores the EP300 gene rs20551 polymorphism and systolic stroke（IS）phlegm-blood stasis syndrome and coronary heart disease（coronary heart disease）phlegm-blood stasis syndrome,as well as its blood pressure,blood glucose,blood lipids,coagulation indicators,and C-reactive protein（CRP）.,Uric acid（UA）,homocysteine（Hcy）association study.Methods:This case-control study divided 550 patients with IS phlegm and blood phlegm-blood stasis syndrome in Han people of Guangxi,China（306men and 244 women）,550 patients with coronary heart disease phlegm and blood phlegm-blood stasis syndrome（316 men,234 women）,and matching corresponding 550 healthy controls（293 males and 257 females）.The genotyping of the rs20551 polymorphic site of EP300 gene was performed using Massarray SNP technology.Real-time quantitative PCR reactions（q RT-PCR）were used to measure the expression level of EP300 gene m RNA in venous blood of patients with IS and CHD phlegm-blood stasis syndrome.Statistical analysis was performed using SPSS 16.0 and PLINK software.Result:1.Hardy-Weinberg Equilibrium（HWE）test and genotype distributionThe genotype frequency distribution of the rs20551 polymorphism of the EP300 gene conforms to Hardy-Weinberg equilibrium（P_H=0.280）;the frequency distribution of the GG,GA,and AA genotype of the EP300 gene rs20551 polymorphism in IS phlegmblood stasis syndrome or coronary heart disease respiratory syndrome There was no significant difference with specific grades（all P>0.05）.2.Association analyses of EP300 rs20551 and susceptibility of phlegmblood stasis syndrome with IS and CHDThere was no significant association between EP300 gene rs20551polymorphism and IS phlegm stasis syndrome and CHD phlegm stasis syndrome（all P>0.05）.3.Correlation between EP300 rs20551 and clinical indicators of patients with IS phlegmblood stasis syndrome and patients with CHD phlegmblood stasis syndrome（1）EP300 rs20551 and serum VLDL stealth model in patients with IS phlegmblood stasis syndrome(recessive mode:P_adj<0.001),APTT(Additive model:P_adj=0.035;Dominant model:P_adj=0.042),PTA(Additive model:P_adj=0.014;Dominant model:P_adj=0.024)Levels were significantly correlated;further dimensional hierarchical analysis found that rs20551 was more than And CRP in the serum of male patients with phlegm and stasis syndrome(Additive model:P_adj=0.005;Dominant model:P_adj=0.005)level was significantly correlated;rs20551 polymorphism was associated with VLDL in the serum of female patients with phlegm stasis syndrome(Additive model:P_adj=0.008;Stealth model:P_adj<0.001),PTA(Additive model:P_adj=0.044),TT(Additive model Type:Padj=0.008;Dominant model:P_adj=0.002)levels are significantly correlated.（2）EP300 gene rs20551 polymorphism and CHP in patients with phlegm and stasis syndrome(invisible model:P_adj=0.047),APTT(Recessive model:P_adj=0.018),DD(Recessive model:P_adj=0.000),PLT(Additive model:P_adj=0.013;Dominant model:P_adj=0.025),TT(Additive model:P_adj=0.002;Recessive model:P_adj<0.001),Hcy(Additive model:P_adj=0.007;Dominant model:P_adj=0.007)level was significantly correlated;further gender distribution analysis found that rs20551 polymorphism in male phlegmblood stasis syndrome In patients with DD(Additive model:P_adj=0.007;Recessive model:P_adj<0.001),PLT(Additive model:P_adj=0.044;Dominant model:P_adj=0.039),CRP(Recessive model:P_adj=0.049),Hcy(Additive model:P_adj=0.002;Dominant model:P_adj=0.002)the level correlation is statistically significant;rs20551 polymorphism and APO-A Additive model in female patients with phlegmblood stasis syndrome:(Additive model:P_adj=0.049;Dominant model:P_adj=0.031),VLDL(Additive model:P_adj=0.039;Dominant model:P_adj=0.026),APTT(Invisible model:P_adj<0.001),PLT(invisible model:P_adj=0.012),TT(Additive model:P_adj=0.001;Recessive model:P_adj<0.001)were significantly correlated.4.Comparison of EP300 m RNA expression level in phlegm-blood stasis syndrome with IS and CHD and controlThe m RNA expression level of IS phlegm and stasis syndrome was significantly higher than the above,which was statistically significant（P<0.001）,while the m RNA expression level of CHD phlegm and stasis syndrome was significantly higher than the above,which was statistically significant（P<0.001）.Conclusions:（1）EP300 gene expression level may be related to the occurrence of phlegmblood stasis syndrome of large-scale stroke and coronary heart disease,and EP300 gene may be a risk gene shared by both diseases;（2）rs20551 polymorphism of EP300 gene may be involved Lips metabolism,blood coagulation function and inflammatory response of phlegm and stasis syndrome of sexual stroke,coronary heart disease and phlegm and stasis syndrome.