| OBJECTIVE: The aim of this study was to investigate long-coding noncoding RNA(lncRNA)H19 single nucleotide polymorphisms rs217727,rs4929984 with IS phlegm-stasis syndrome and CHD phlegm-stasis syndrome susceptibility to genetic association studies.Methods: A total of 1650 subjects were included in the study,including 550 patients in the IS phlegm-stasis syndrome group,550 patients in the CHD phlegm-stasis syndrome group,and 550 patients in the control group.General information and clinical data of all subjects were collected by means of a unified questionnaire,and Sequenom MassARRAY technique was used for genotyping of lncRNA H19 single nucleotide polymorphisms Rs217727 and Rs4929984.The levels of lipids(APO-A1,APO-B,VLDL,HDL-C,LDL-C,TC,TG)in serum of patients with IS phlegm-stasis syndrome were detected by HITACHI Hitachi 7600 automatic biochemical analyzer;GOD-PAP method was used for detection.Blood glucose FPG,P2 hPG levels;enzyme kinetics colorimetric detection of Hcy levels;detection of CRP levels by immunoturbidimetry;detection of plasma coagulation function indicators(APTT,DD,FIB,INR,PT,PLT,using the French StAgOCapact blood coagulation instrument)TT)level.SPSS16.0 for windows software and PLINK software were used for statistical analysis.Real-time quantitative PCR was used to detect mRNA expression levels of lncRNA H19.RESULTS: 1.The genotype distribution frequency of LncRNA H19 single nucleotide polymorphisms rs217727 and rs4929984 in the control group accorded with the Harvar's equilibrium law(P>0.050);The genotype frequencies of the two polymorphic loci of lncRNA H19 were not significantly different between the IS group and the control group(P>0.050).The genotype frequencies of the two polymorphic loci of lncRNA H19 were not significantly different between the CHD sputum group and the control group(P>0.050).Analysis by sex: In women,the genotype frequency of rs4929984 was statistically significant(P<0.050).2.There was no significant correlation between the rs217727 and rs4929984 polymorphisms of lncRNA H19 and the susceptibility to IS phlegmstasis syndrome(P>0.050).There was no statistically significant association between these two loci polymorphisms and susceptibility to CHD phlegmstasis syndrome(P>0.050).Analysis by sex: rs4929984 of lncRNA H19 was significantly associated with female CHD phlegm-stasis syndrome susceptibility(P<0.050),but there was no significant correlation with susceptibility to male CHD phlegm-stasis syndrome(P>0.050).3.Quantitative trait association analysis results: LncRNA H19 single nucleotide polymorphisms rs217727,rs4929984 and IS phlegm-stasis syndrome and CHD phlegm-stasis syndrome quantitative traits analysis results: SNP Rs 217727 was significantly associated with INR levels in patients with IS phlegm-stasis syndrome(P < 0.050)and was significantly associated with VLDL,P2 hPG and CRP levels in patients with CHD phlegm-stasis syndrome(P < 0.050).SNP rs4929984 was significantly associated with DBP levels in patients with IS phlegm-stasis syndrome(P < 0.050)and was significantly associated with INR,PTA and CRP levels in patients with CHD phlegm-stasis syndrome(P < 0.050).Stratified by sex: rs217727 was significantly associated with APTT,INR,and PTA levels in male patients with IS phlegm-stasis syndrome(P < 0.050),and was significantly associated with Hcy levels in female IS phlegm-stasis syndrome patients(P<0.050),and was significantly correlated with VLDL,INR,PTA,P2 hPG and CRP levels in male CHD phlegm-stasis syndrome patients(P<0.050),and was significantly correlated with LDL-C and PLT levels in female CHD phlegm-stasis syndrome patients(P<0.050).SNP rs4929984 was significantly associated with serum APO-A1,PTA,DBP,and UA levels in male patients with IS phlegm-stasis syndrome(P<0.050),and was significantly associated with FIB and INR levels in male CHD phlegm-stasis syndrome patients(P<0.050),and was a significant correlation between HDL-C and INR levels in women CHD phlegm-stasis syndrome patients(P<0.050).4.Compared with the normal control group,the expression levels of lncRNA H19 in the IS phlegm-stasis syndrome group and the CHD phlegmstasis syndrome group were significantly up-regulated(P<0.001).Conclusion: LncRNA H19 genetic polymorphism may affect the risk of CHD phlegm-stasis syndrome in women.Peripheral blood expression levels of LncRNA H19 may have an effect on the occurrence of ischemic stroke phlegm-stasis syndrome and coronary heart disease phlegm-stasis syndrome. |
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