Association Between The Single Nucleotide Polymorphism(SNP)of Oxytocin Receptor(OXTR)Gene And Autism Spectrum Disorders(ASD):a Meta-analysis

Background:Autism spectrum disorder?Autism Spectrum Disorder,ASD?is a mental illness with increasing prevalence,high disability rate and high risk of suicide.At present,although its researches involve many fields,its etiology and mechanism are still unclear,genetic factors may be risk factors for ASD.Single nucleotide polymorphisms?single nucleotide polymorphism,SNP?of the oxytocin receptor?oxytocin receptor,OXTR?gene are associated with a variety of diseases,but the effect size of the OXTR gene SNP involved in the association study is not consistent,making it unclear whether the OXTR gene is truly relevant to the ASD.Objective:To explore whether OXTR gene SNP is associated with ASD by meta analysis,and to clarify which specific SNPs have a significant impact on ASD,to provide evidence-based medical evidence for genetic background and prevention strategies.Methods:Using computer to retrieve case-control studies on the relevance of OXTR gene SNP to ASD published in PubMed?Cochrane Library?Embase?CNKI full-text database?China Knowledge Network?,the Weip Chinese Science and Technology Journal Database?Weip Database?and the Wanfang Knowledge Service platform?Wanfang Database?,without limiting language and language,the retrieval time is from the database to December 2019.the retrieved literature was screened according to the set inclusion criteria and exclusion criteria.stata15.1software was used for meta analysis,including heterogeneity test,combined effect quantity and publication bias evaluation.Results:1.Finally included 5 high-quality English literatures for meta analysis.All the studies were case-control studies,involving a total number of 1169 cases and a total number of 1778 controls.2.Sensitivity analysis:The results of rs53576 allele model?G vs A?and rs53576 heterozygous gene model?GA vs AA?were not reliable in the study of 2016 Milton^[40]et al.3.SNP rs2254298 of OXTR gene:There were no statistically significant difference?P>0.05?in all gene models.The results do not support the correlation between SNP rs2254298 of OXTR gene and ASD.After subgroup analysis by race,there was statistically significant difference?P<0.05?in the allele model?A vs G?group?OR=1.35,95%CI=[1.07-1.70]?in asian population.There were no statistically significant difference?P>0.05?in the rest of gene models and subgroup analysis of population.The results showed that the risk of ASD in asian population with OXTR gene SNP rs2254298“G”alleles was lower than who with“A”alleles.4.SNP rs53576 of OXTR gene:There was were not statistically significant?P>0.05?in all gene models.The meta analysis was carried out again after excluding the study of 2016 Milton^[40]et al.it was found that the difference was statistically significant?P<0.05?in the rs53576allele model group?G vs A??OR=0.80,95%CI=[0.67-0.94]?.The results showed that the"G"allele of the OXTR gene may be a protective factor for ASD.After subgroup analysis by race,the difference was still not statistically significant?P>0.05?.5.SNP rs1042778,rs2301261 and rs237885 of OXTR gene:There were no statistically significant difference?P>0.05?in the rs1042778allele model?T vs G?group?OR=0.95,95%CI=[0.82-1.11]?,the rs2301261allele model?T vs C?group?OR=1.00,95%CI=[0.62-1.63]?and the rs237885 allele model?G vs T?group?OR=0.86,95%CI=[0.72-1.04]?.After subgroup analysis by race,there was still no statistically significant difference?P>0.05?.The results showed that there was no significant relationship between OXTR gene SNPs rs1042778,rs2301261 and rs237885 and ASD.6.Heterogeneity test:The I² values of the rs2254298 allele model?A vs G?,the rs53576 recessive gene model?GG vs GA+AA?,the rs53576superdominant gene model?GG+AA vs GA?and the rs2301261 allele model?T vs C?were respectively 65.8%,66.6%,75.4%and 59.4%,indicating that there was heterogeneity in the study of these models,through subgroup analysis to continue to explore the source of heterogeneity,found that some of the heterogeneity of the studies came from the asian population,using random effect model,other gene models using fixed effect model.Conclusion:1.Subgroup analysis by race showed that the risk of ASD in caucasian populations with OXTR gene SNP rs2254298“G”alleles is lower than who with“A”alleles.2.The“G”allele of SNP rs53576 of the OXTR gene may be protective factors for developing ASD.3.There was no significant relationship between OXTR gene SNPs rs1042778,rs2301261 and rs237885 and ASD.4.Due to the limited number of studies included in this paper,some studies have obvious heterogeneity,some research results are unstable,some research literature quality is low,there are some limitations,more large samples,methodology and report high-quality genetic association studies are still needed to move further and verify these results in the future.