Mechanism Of Anti-depression And Neuroprotective Effects Of Curcumin On Mice With Spinal Cord Injury

ObjectiveTo study the neuroprotective effect of curcumin(CCM)after spinal cord injury(SCI)in mice,and to investigate whether its antidepressant effect is related to extracellular signal-regulated kinase(ERK)protein and the expression of Brain Derived Neurotrophic Factor(BDNF).Materials and methodsA modified aneurysm clip device model~1 was used to simulate the occurrence of spinal cord injury.Healthy mice were randomly divided into three groups:spinal cord injury control group((SCI+Veh group),spinal cord injury intervention group(curcumin intervention treatment after spinal cord injury,(SCI+CCM)group),and sham operation group(simple removal of lamina without Spinal cord injury,Sham group),in which the(SCI+CCM)group was given curcumin(200mg/kg)gavage intervention therapy at 30 minutes after spinal cord injury,once a day for 7 days,while the(SCI+Veh)group and the Sham group were given the same amount of physiological saline Gavage.We evaluated curcumin neuroprotective effect through BMS(basso mouse scale)score,slanted plate test,PI(propidium iodide staining)staining,FJB(fluoro-jade b)staining,spinal cord water content determination,superoxide dismutase(SOD)measurement,malondlaldehyde(MDA)measurement.Sugar water preference test and tail suspension test were used to evaluate the antidepressant effect of curcumin,and we explore its mechanism of antidepressant effect through BDNF and ERK protein expression.Results1.Long-term curcumin treatment significantly improved depressive behavior in mice with spinal cord injury.Compared with the SCI+Veh group,SCI+CCM group significantly reduced the immobility time of the mice(p<0.05)and significantly increased the percentage of sucrose partiality of the mice(p<0.05).2.Long-term curcumin treatment increased expression of ERK protein and BDNF protein in mouse amygdalaCompared with the SCI+Veh group,SCI+CCM group significantly increased the expression of ERK protein and BDNF protein in SCI mice(p<0.05).3.Curcumin treatment has anti-inflammatory effects on mice with spinal cord injuryCompared with the SCI+Veh group,the SOD level in the SCI+CCM group was significantly increased(p<0.05),and the MDA level was significantly decreased(p<0.05),and the spinal water content was significantly decreased(p<0.05).4.Curcumin treatment of spinal cord injury reduces cell necrosis and apoptosisCompared with the SCI+Veh group,PI staining showed that necrotic cells in the area around the spinal cord injury of the SCI+CCM group were significantly reduced(p<0.05);FJB staining showed that positive cells represented neuronal degeneration and necrosis.The number of stained positive cells in the mice in the group was significantly reduced(p<0.05).5.Curcumin treatment of spinal cord injury improves hindlimb motor functionCompared with the SCI+Veh group,SCI+CCM group significantly increased the BMS score and the slope of the level(p<0.05).Conclusion1.Mice will show depressive behavior after spinal cord injury;2.Curcumin treatment can effectively prevent depressive behavior in mice after spinal cord injury;3.The antidepressant effect of curcumin in preventing depression after spinal cord injury may be partially mediated by BDNF protein;4.Curcumin treatment has neuroprotective effect on mice with spinal cord injury.