Study On The Changes Of Biological Behaviors In Cal-27 Cell Line After Depression Of HDAC2 By RNAi

Objective:In our previous studies,increased HDAC2 expression was found in some pathological specimen of OSCC.We also found HDAC2 get the highest score on MaximalClique Centrality(MCC)among the screened 63 hub genes related to TSCC in oral cancer’s mice model,which shows evidence that HDAC2 plays a significant role in oral cancer.In this study,we aim to explore the significance of HDAC2 expression in the human OSCC cell lines and the influence of HDAC2 expression on the biological behaviors of OSCC cell lines both in vitro and vivo.MethodsThe lentiviral vector haboring sequence interferences the HDAC2 was constructed and transfected into human Cal-27 tongue cancer cell line to obtain HDAC2-shRNA-Cal-27 and control-shRNA-Cal-27.The efficiency of transfection was observed under fluorescence microscope.We screened them with the puromycin to construct Cal-27 cell expressed shRNA stabely.Detected the expression of HDAC2 at mRNA and protein levels by qRT-PCR and Western-blot.Cell proliferation was evaluated by CCK-8 assay and colony formation assay,while cell migrationwas evaluated by wound healing assay,and the ability of invasion was detected by cell invasion assay.Results1.Construction of OSCC Cal-27 cell lines with Low-level expression of HDAC2shRNA interference against HDAC2 was used in the Cal-27 cell line to decrease the HDAC2 expression level.The HDAC2 expression level was verified by Western blot and real-time PCR.2.Decreased abilities of cellular proliferation and colony formation were found in the Cal-27 cells when interfered by shRNA against HDAC2.3.Decreased abilities of cellular invasion and were found in the Cal-27 cells when interfered by shRNA against HDAC2,while the abilities of cellular migration were not found different.conclusions:Increased HDAC2 expression is common in OSCC cell lines.HDAC2 improved the cellular proliferation in OSCC cell lines,which acts like a tumor promoted gene.After down regulation of HDAC2,the OSCC cells have a slower cellular proliferation and invasion.