The Study On Imbalance Of Peripheral Blood CD8+T Lymphocyte Subsets And The Correlation Between The Levels Of CD8+T Lymphocyte Subsets And IgE In Patients With Asthma

Objective:With the advent of modern industrialization in the mid twentieth century,the incidence of bronchial asthma has increased dramatically.This not only affects the quality of life and hinders work and learning seriously in patients with asthma,but also aggravates the patient's financial burden.Studies show that repeated inflammatory-injury caused by immune dysfunction plays an extremely important role in the pathogenesis of asthma.Previous studies showed that CD4+T lymphocyte subsets and their pro-inflammatory cytokines play an important role in asthma allergic inflammation,the role of subpopulations of CD8+T lymphocyte in asthma has long been neglected yet.In recent years,some studies have found that CD8+T lymphocyte subsets can also play a role in the pathogenesis of asthma,but the specific mechanism still needs to be further explored.By studying the imbalance of peripheral blood CD8+T lymphocyte subsets in patients with asthma.And analysis of the correlation between CD8+T lymphocyte subsets(CD8+CD28+ and CD8+CD28-Tcells)and immunoglobulin E,the correlation between CD8+CD28+Tcells and pulmonary function(FEV1%pred),to further explore the immune regulation mechanism of asthma.To provide new research ideas and basis for future immune intervention in asthma.Methods:1.40 cases of hospitalized patients with acute asthma attack in Qingdao Municipal Hospital from May 2016 to February 2017 have been included into case group and 40 healthy people as the control group for study;2.Flow cytometry was used to detect the levels of T lymphocyte subsets in peripheral blood of healthy control and research groups,including CD3+%,CD4+%,CD8+%,CD4+/CD8+ ratio,CD8+CD28+%,and CD8+CD28-%;3.Determination of serum IgE levels of all subjects by enzyme-linked immunosorbent assay;4.Pulmonary function was used to determine the level of lung function in both groups,including FVC and FEV1%pred;5.To review the level of T lymphocyte subsets,IgE and pulmonary function after two weeks of effective treatment in the case group;6.Observe the change of levels of T-lymphocyte subsets,IgE and pulmonary function(FEV1% pred,FVC)in the acute asthma attack stage and asthma remission stage in the case group and compare with the control group;Analysis of the correlation between CD8+CD28-Tcells and immunoglobulin E,the correlation between CD8+CD28+T cells and pulmonary function(FEV1%pred)in the acute attack stage of asthma.Results:1.This study completed successfully that all participants without lost,exit and deaths.2.The levels of CD3+ T lymphocytes were higher in the case group than in the control group,but the difference was not statistically significant(P>0.05).The levesl of CD4+ T lymphocytes and the ratio of CD4+/CD8+ in the case group were significantly higher than those in the control group(P<0.05),and asthma exacerbation higher than asthma remission(P<0.05).The levels of CD8+T lymphocyte were lower in the case group than in the control group,but the difference was not statistically significant(P>0.05).The levels of CD8+CD28+ T cells in the asthma exacerbation,asthma remission,and control group were(12.09±3.78)%,(3.05±1.76)%and(1.20± 0.59)%,respectively.The case group was higher than the control group(P <0.05).In the case group,the exacerbation of asthma was higher than asthma remission(P<0.05).The levels of CD8+CD28-T cells in the asthma exacerbation,asthma remission and control groups were(9.92±6.05)%,(17.77±3.36)%and(22.23±3.31)%,respectively.The case group was lower than the control group(P<0.05).In the case group,the exacerbation of asthma was lower than asthma remission(P<0.05).3.The levels of IgE in asthma exacerbation,asthma remission,and the control group were(389.02±142.47)IU/ml,(106.57±22.44)IU/ml and(46.20±18.17)IU/ml,respectively.The case group was significantly higher than the control group(P<0.05).The exacerbation of asthma was lower than asthma remission(P<0.05).4.The level of pulmonary function(FEV1% pred)in the case group was lower than the control group.In the case group,the asthma exacerbation was significantly lower than asthma remission(P<0.05).5.In the asthma exacerbation,there was no significant correlation between the level of CD8+CD28+ T cells and IgE(r=0.10,P>0.05).There was a negative correlation between the level of CD8+CD28-T cells in peripheral blood and the expression of IgE(r=-0.814,P<0.05).There was a negative correlation between the level of CD8+CD28+ T cells and lung function(FEV1%pred)(r=-0.507,P<0.05).Conclusions:1.Patients with bronchial asthma have imbalance of CD8+T lymphocyte subsets,elevated CD8+CD28+T cell levels,and decreased CD8+CD28-T cell levels.2.The subpopulation of T lymphocytes in patients with remission of asthma is still an imbalanced state,but it is better than in the acute attack period,suggesting that the recovery of imbalance T lymphocyte subsets in asthmatic patients is a chronic proess that lags behind clinical symptoms.3.CD8+CD28-T cells are negatively correlated with IgE levels in asthma exacerbation.CD8+CD28-T cells may participate in the chronic airway inflammation of asthma by indirectly regulating IgE levels.4.The level of CD8+CD28+ T cells was negatively correlated with FEV1%pred in asthma exacerbation,suggesting that the level of CD8+CD28+T cells is closely related to the degree of airflow limitation in the acute attack of asthma patients.