To Explore The Effect Of Lentivirus-mediated Differentiation Of BMP-2 And VEGF-165-transfected Goat BMSCs Into Osteogenesis

Objective:To explore the effect of lentivirus-mediated differentiation of BMP-2 and VEGF-165-transfected goat BMSCs into osteogenesis.Methods: the primary goat bone marrow mesenchymal stem cells were cultured by whole bone marrow adherent method,and the target gene was transfected and mediated by virus to study the feasibility of goat BMSCs as the gene co-transfer target cell,as well as the biological changes of cells in vitro,such as culture conditions,proliferation,differentiation into osteogenesis after transfection etc..Results:the expression of BMP-2mRNA was not significantly different from the group of BMP-2,and also the expression of VEGF-165 mRNA was not significantly different from the group of VEGF-165.The expression of OCN mRNA in Lv-BMP2-VEGF165 transfection group was higher than that in single gene transfection group(P < 0 05),and BMP2 and VEGF165 proteins were only highly expressed in Lv-BMP2-VEGF165 group.The expression of OCN protein in Lv-BMP2-VEGF165 group was significantly higher than that in other groups(p < 0.01),and the activity of alkaline phosphatase in Lv-BMP2-VEGF165 transfection group was significantly higher than other groups.Conclusion:BMP2,VEGF165 and BMP2/VEGF-165 can be successfully transferred into goat bone marrow mesenchymal stem cells and can be stably expressed for a long time.The synergistic effect of BMP2/VEGF-165 could promote the production of alkaline phosphatase,the synthesis ofosteocalcin and make the cells develop into osteogenesis.