Differential Expression Of Hsa_circ₀020397 In Nasopharyngeal Carcinoma And Its Significance

Methods:1.Tissue samples from patients with nasopharyngeal carcinoma were selected as research objects.TissueObjective:In this study,high-throughput sequencing technology was used to obtain differential expression profiles of circRNA in nasopharyngeal carcinoma and nasopharyngitis tissues.Through the GO enrichment and KEGG enrichment analysis of differentially expressed circRNA,the functional classification and related pathways of differentially expressed circRNA were further understood.The differentially expressed hsa_circ₀020397 was screened for verification by parallel real-time fluorescent quantitative PCR?qPCR?.The analysis showed that hsa_circ₀020397 was expressed in nasopharyngeal carcinoma tissues,and its value as a new target for the diagnosis of nasopharyngeal carcinoma was determined.samples from nasopharyngeal carcinoma and chronic nasopharyngeal inflammation were collected,RNA was extracted,and total RNA purity was detected.High throughput sequencing was used to detect the differences in the expression levels of nasopharyngeal carcinoma and nasopharyngitis tissues,and to obtain the differential expression profiles of circRNA in nasopharyngeal carcinoma tissues and nasopharyngeal chronic inflammation tissues.The sequencing results were analyzed by bioinformatics,and the differentially expressed circRNA and its target genes obtained from preliminary screening were analyzed by GO and KEGG enrichment analysis,and the functional classification and related pathways of differentially expressed circRNA were analyzed.2.The differential expression profile was verified by qPCR test,and the relative expression level of hsa_circ₀020397 in 30 cases of nasopharyngeal carcinoma and 20 cases of chronic nasopharyngitis was detected.Graphpad Prism5 software was used to analyze and graph the data.Result:1.CircRNA high-throughput sequencing test results:A total of 796 circRNAs showed statistically significant differences in the expression of circRNAs in nasopharyngeal carcinoma and chronic nasopharyngitis tissues?P < 0.05,log2?absolute value of foldchange?? 1?,including 232 circRNAs whose expression was up-regulated and 564 circRNAs whose expression was down-regulated.2.Results of Gene Ontology?GO?Analysis of circRNA Target Genes:The GO enrichment analysis of the target genes differentially expressed circRNA showed that the cell projection assembly was the highest degree of enrichment in biological process?BP?,and centrosome in cell component?CC?,and GTPase binding in molecular function?MF?.3.Analysis of KEGG enrichment pathway of circRNA target gene:We conducted KEGG enrichment analysis on the target genes differentially expressed circRNA to obtain 15 relevant signaling pathways,among which the more critical pathways were human t-cell leukemia virus 1 infection and MAPK signaling pathway.4.Verification results of differential expression profile by real-time fluorescence quantitative PCR experiment:One upregulated hsa_circ₀020397?hsa_circDOCK1₀24?with significant change?with large difference?was selected to verify its expression in tissue samples of nasopharyngeal carcinoma and nasopharyngeal chronic inflammation,and the results confirmed that the upregulated trend of hsa_circ₀020397 was consistent with the sequencing results.Conclusion:1.Differential expression profiles of circRNAs in nasopharyngeal carcinoma and chronic nasopharyngitis were obtained.2.Hsa_circ₀020397 was confirmed to be highly expressed in nasopharyngeal carcinoma by qPCR,which is expected to become a new target for the diagnosis of nasopharyngeal carcinoma.