Correlation Between Neurogenic Inflammation And LPRD

Objective : Analyze the degree of submucosal inflammatory infiltration and expression of SP,NK-1R,PGP9.5,and IL-8 in the throat of animal models of laryngopharyngeal reflux disease.To investigate the correlation between laryngopharyngeal reflux disease and neurogenic inflammation.Methods:Forty healthy albino male guinea pigs were randomly divided into an experimental group and a control group,each with 20 animals.The laryngopharyngeal reflux animalmodel was constructed by exogenous method.Guinea pigs are anesthetized by intraperitoneal injection of 1% sodium pentobarbital and fixed on a flat plate.The epidural anesthesia tube is intubated through the mouth to the middle and upper esophagus(about 5-6cm from the mouth angle),and the gastric simulation fluid and physiological saline were perfused at a rate of 8 drops/min.Forty guinea pigs were sacrificed after 14 days of perfusion.HE staining was performed to detect the infiltration degree of leukocytes in the laryngeal mucosa.Quantitative real-time PCR(qPCR)was used to detect the gene expression of SP,NK-1R,PGP 9.5 and IL-8,respectively.Western blot was used to detect the expression of NK-1R,PGP 9.5,and the expression of intergroup genes and proteins was statistically analyzed.Results:1.The infiltration of submucosal leukocytes increased in the experimental group compared with the control group,and the difference was statistically significant(P(27)0.05).2.There was no significant difference in the expression of SP mRNA between groups(P(29)0.05);NK-1R,IL-8,and PGP9.5 mRNA expression in the experimental group were all up-regulated compared with the control group(6.32?3.34?2.27),and the difference was statistically significant(P(27)0.05).3.The expressions of NK-1R and PGP9.5 in the experimental group were up-regulated compared with those in the control group,and the differences were statistically significant(P(27)0.05).Conclusion : Laryngopharyngeal reflux disease is significantly related to neurogenic inflammation.The upregulation of NK-1R expression in the experimental group indicates that neurogenic inflammation may be involved in the development of laryngopharyngeal reflux disease,and the inflammation of the throat is enlarged by overexpression of NK-1R;The up-regulation of IL-8 expression in the experimental group suggested that the up-regulation of NK-1R expression was mainly full-length.The upregulation of the expression of PGP9.5 in the throat of the experimental group confirmed the change of the sensory nerve fibers in the laryngopharyngeal.The high sensitivity of the laryngopharyngeal in patients with laryngopharyngeal reflux disease may be related to the expression of sensory nerve fibers.