| Background:Photoaging caused by prolonged skin exposure to ultraviolet(UV)light is the most important cause of exogenous aging.The typical histological changes are the disability of various types of stromal cells and the degradation and ratio imbalance of various types of fibrin.Adipose stem cell matrigel(ECM/SVF-gel)is a product of fat processed through a series of mechanical processes.It contains a large number of concentrated functional stem cells and extracellular matrix.It is currently mainly used as an autologous filler and is used in the field of cosmetic surgery.Due to its delicate texture,it overcomes the shortcomings of traditional fat transplantation that cannot be used for precise needle injection.The purpose of this study was to evaluate the possibility and effectiveness of SVF-gel dermal injection against skin photoaging and to initially explore its mechanism of action.Methods:Nude mouse skin was irradiated with increasing UVB energy for eight weeks,four times a week,to establish a nude mouse photoaging model.In the treatment group,SVF-gel intradermal injection prepared with human fat was used.At the same time,the clinical control group was treated with the same volume of Coleman fat transplantation and external retinoid acid smear.The blank control group was treated with phosphate-buffered saline.Evaluation of photoaging skin characterization and collagen composition by wrinkle microphotography,histology,and immunohistochemistry.The number and function of fibroblasts were examined,and the expression of genes related to local collagen synthesis and photoaging in each group was further compared.Results:After UVB irradiation,the skin texture of nude mice was rough,and the depth and width of wrinkles were significantly increased compared with the non-irradiated group,and typical photoaging performances such as thinning of the dermis layer appeared.Microphotographs show that SVF-gel is smaller than Coleman fat particles.After quantification,it is found that the diameter is about 1/10 of Coleman fat,which can be injected intradermally.After 6 weeks of treatment,SVF-gel,Coleman fat,and tretinoin gradually showed different levels of skin repair performance,and the wrinkles and thickness were better than the clinical control group.Histological examination shows that the SVF-gel treatment group can better reconstruct the skin fat layer of mice,increase the density of dermal collagen,and increase the proportion of type I and type III collagen.Through quantitative and functional staining of fibroblasts,it can be seen that although the SVF-gel treatment group cannot significantly stimulate its proliferation,it can increase the secretion of local type I procollagen peptide(PINP)and the concentration of local transforming growth factorβ1(TGF-β1).We also found that SVF-gel contains a large amount of large precursor complex(Large Latent Complex,LLC),which is a precursor peptide complex that covalently binds and stores inactive TGF-β,and its content is more than 3 times that of fat tissue.After SVF-gel treatment,at the level of transcription factors,it can partially reverse the changes of photoaging,which is superior to other treatment groups,and Fibulin-5(Fibulin-5)becomes the matrix protein of SVF-gel which is different from the fat transplantation group.Conclusions:The SVF-gel particles are fine,and the graft can survive in the dermis layer,showing a strong anti-photoaging effect in the mouse UVB irradiation model.SVF-gel increases the local concentration of TGF-β1 and stimulates fibroblasts to produce PINP,thereby increasing type Ⅰ collagen,thickening the dermis layer and improving fine lines.This study presents new possibilities of SVF-gel as a fat product in the field of anti-aging treatment,suggesting that SVF-gel has the potential to become a powerful anti-wrinkle tool that combines"filling" and "anti-aging". |
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