Effects Of Cell Adhesion Molecule Related Genes SELE,COL1A1,ITGAV And CAECAM1 On Allergic Rhinitis

Objective With the change of environmental factors,the global incidence of allergic diseases has increased year by year,and allergic rhinitis has received more and more attention in China.With the continuous understanding of the pathogenesis of AR,the role of T helper cells and related secretion factors secreted has been gradually recognized in the development of AR.Cell adhesion molecules are a class of molecules that mediate contact and binding between tract cells or between cells and stroma.Through literature review and previous research,we found that the expression profile of cell adhesion molecules is increased in patients with allergic rhinitis.In this study,the expression of cell adhesion molecules in nasal mucosal wipes from patients with allergic rhinitis and healthy volunteers was analyzed to investigate whether it has a certain role in the process of allergic rhinitis disease.Methods In the previous research,the expression profile chip was used to select four adhesion molecules,including SELE,ITGAV,COL1A1 and CEACAM1 gene.Nasal mucosal scrapes were collected from 24 patients with Allergic rhinitis(AR)and 17 healthy volunteers diagnosed in the outpatient clinic of Zhujiang Hospital,Southern Medical University from March 2019 to June 2019.The visual analogue score(VAS)、general condition of the patient and allergen status of allergic rhinitis symptoms were recorded in the group.The scraped material was divided into three parts and stored separately.One of them was stored in RNAlater-80 degree refrigerator,For the determination of mRNA levels;a 4%paraformaldehyde solution stored at 4 degrees for immunofluorescence measurement.Adhesion molecules SELE,ITGAV,COL1A1 and CEACAM1 mRNA level;Nasal scrape in 4%paraformaldehyde solution for cell smears,and further immunofluorescence to determine the expression of corresponding proteins.GraphPad Prism 6 statistical software was used for statistical analysis.The test with Welch’s correction test analyzed the expression levels of SELE,ITGAV,COL1A1,and CEACAM1 genes and immunofluorescence.P<0.05 was considered statistically significant.Results All the enrolled patients and healthy control volunteers filled out the questionnaire to collect the basic information of the patients.Of the 24 patients,20 were male and 4 were female,aged 16 to 43 years,with an average age of(32.4±8.4)years.Among the healthy volunteer control group,there were 10 males and 7 females,aged 18 to 51 years,with an average age of(37.1 ± 9.7)years.After rubbing the mucous membranes,a puncture test was performed on all the participants and the results of the dust mites and house dust mites were counted.3 of them were allergic to dust mites,4 were allergic to house dust mites,and 17 were allergic to dust mites and house dust mites.The qRT-PCR results showed that the expression of SELE gene was significantly increased in patients with allergic rhinitis compared with the control group(P=0.030),and there was no significant difference in the expression of the ITGAV,COL1A1,and CEACAM1 genes in the experimental group and the healthy control group(P values were 0.364;0.423;0.392).The immunofluorescence results also showed that the expression of SELE gene in patients with allergic rhinitis had an upward trend compared with the healthy control group(P=0.053);there was no significant difference between the patients with allergic rhinitis and the healthy control group in ITGAV,COL1A1 and CEACAM genes(P=0.056;0.272;0.563).The nasal symptoms score,non-nasal eye symptom score,and VAS syndrome score of patients with allergic rhinitis were significantly higher than those of non-allergic rhinitis patients,and the difference was statistically significant(P<0.05).There was no significant correlation between ocular symptom scores and AR.There was no significant correlation between the expression of SELE gene and the severity of symptoms(P>0.05).Conclusion The SELE gene is highly expressed in the nasal mucosa of AR patients.This gene may be a potential gene locus for the development of allergic rhinitis and is closely related to the development of AR.However,there is no evidence to support the correlation between SELE and the severity of AR symptoms.The mechanism of its role in the development of allergic rhinitis needs further experimental research.CEACAM1,ITGAV,and COL1A1 were not significantly related to the pathogenesis of AR.