| Objective: The purpose of this study was to investigate the effects of atmospheric fine particulate matter(PM2.5)on the symptoms of AR rats,the ciliary state on the nasal mucosal,and the expression of nasal mucosal inflammatory cytokines and adhesion molecules.Methods:30 healthy female BALB/c mice were selected and randomly divided into six groups(6 animals per group): the negative control group(NCgroup),an ovalbumin(OVA)induced AR model(AR group),and three PM2.5-exacerbated AR groups exposed to three different concentrations: 30,300,and 3000?g/ml of PM2.5 in ARL,ARM,and ARH groups.Sneeze and nose-scratching events were observed.The expressions of ICAM-1(intercellular adhesion molecule)and VCAM-1(vascular cell adhesion molecule-1),IL-4,IL-10,IL-13,TSLP and IL-6 in mouse nasal mucosa were determined by Western blot(WB)and immunohistochemistry.The changes of nasal mucosal epithelial cell cilia in each group were observed by sem.SPSS 21.0 is used to statistical analysis,The comparison between the two groups whose measurement data followed a normal distribution was verified by use of a t test.P<0.05 represented statistical significance.Results:In regards to the experimental groups,there were more than 3.3points following administering of the OVA nasal instillation,indicative of the AR rat model establishment.In the course of the experiment the rats were stimulated with various concentrations of PM2.5,the mice in the control group nose scratching and sneezing occasionally.AR group and PM2.5 low concentration group(ARL),middle concentration group(ARM),high concentration group(ARH)mice showed different degrees of AR symptoms.And with the increase of PM2.5 concentration,the sy-mptoms have aggravated,the model group has statistical significance compared with the control group(P<0.05),Comparisons made to the low-concentration group portrayed evidence that was also observed in the rats in the middle concentration and highconcentration groups(both P<0.05).However,no significant differences were observed in the average sneeze time between rats in the middle-concentration group and tho-se in the high-concentration group(P<0.05).Immunohistochemical analys-is showed that IL-4?IL-13?TSLP?IL-6 ICAM-1 and VCAM-1 were positively expressed in different degrees in the AR group and different concentrations of PM2.5 in the nasal mucosa epithelial cells.The intern-al staining was lighter or occasionally individual positive staining,and each index was strongly positive in the ARM and ARH groups(P<0.05);While IL-10 was positively expressed in the nasal mucosal epithelial cells of the control group,but decreased in the AR group and different concentrations of PM2.5 groups.Among them,high concentration of PM2.5 wasstained by light,difference is significant(P<0.05).Densitometric analysis of IL-4,IL-13,TSLP,IL-6,ICAM-1 and VCAM-1 in the AR group and different concentrations of PM2.5 group of nasal mucosa epit-helialcells significantly increased expression trend,Compared with the control group,the difference is significant(P<0.05),However IL-10 sho-wed a gradually weakening expression trend.The results of Western blotconfirmed the above results.The cilia of nasal mucosal epithelial cells of mice in the normal group were orderly arranged under electron micr-oscope,while those in the sensitization group were slightly swollen com-pared with those in the normal group,but the cilia were still orderly arranged.The cilia in the PM2.5 exposure group were disorderly arrange-d,and the cilia were even adherent and fell off.Conclusion:1.PM2.5 exposure can aggravate rhinitis symptoms in mice with allergic rhinitis.2.The expression levels of IL-4,IL-13,TSLP,IL-6,icam-1 and vcam-1 in nasal epithelial cells of AR mice exposed to PM2.5 increased,while the expression levels of il-10 decreased,showing a dose-response relationship.3.PM2.5 can damage the surface cilia of nasal mucosa epithelial cells,causing adhesion and lodging,it may have some impact on its function. |
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