Correlation And Clinical Significance Of VEGFR2 And EGFR Expression In Gastric Cancer

Objective:Angiogenesis plays an important role in the pathological progression of gastric cancer,but the value of antivascular therapy for advanced gastric cancer remains unclear.The purpose of this study was to analyze the expression level of VEGFR2 and EGFR in gastric cancer tissues and its correlation to explore the influence of the two receptors on the clinicopathological characteristics and postoperative recurrence in gastric cancer patients,so as to provide theoretical support for the implementation of molecular targeted therapy in gastric cancer patients.Method:Clinical data of patients admitted to gastrointestinal surgery department and Tumor Surgery Department of the First Affiliated Hospital of Kunming Medical University from January 2016 to December 2016,who underwent radical gastrectomy for gastric cancer for the first time and were pathologically diagnosed as primary gastric cancer after surgery,were collected and enrolled.Finally,the clinicopathological data of 103 patients with gastric cancer meeting the requirements were included for a retrospective analysis.According to the patient's pathological information,the corresponding tumor tissue wax blocks and the corresponding adjacent normal tissue wax blocks were collected from the specimen bank,and the slices were made into white slices by the microtome for future use.By immunohistochemical(immunohistochemical analysis,HTC)method to detect VEGFR2 and EGFR in gastric cancer tissue and the expression level of tissue adjacent to carcinoma,matching the VEGFR2 in tumor tissues and the expression of EGFR level and the patient's gender,age,tumor location,tumor size,TNM stage,tumor infiltration depth,lymph node metastasis,distant metastasis,vascular invasion,tumor differentiation,nerve invasion,survival of the relationship between clinical pathological features.result1.There were 12 negative(-)and 91 positive(+)VEGFR2 expression in cancer tissues,30 negative(-)and 73 positive(+)expression in adjacent tissues.The expression level of VEGFR2 in the adjacent normal tissues was significantly increased(P<0.05).EGFR was negative(-)in 24 cases and positive(+)in 79 cases,and negative(-)and positive(+)in 53 cases and 50 cases in the paracancer group.The expression level of EGFR in gastric cancer tissues was significantly increased,and the difference was statistically significant compared with the corresponding paracancer tissues(P<0.05).2.In gastric cancer tissues,the positive VEGFR2 expression rate was 97.56%when the tumor diameter was larger than 4cm,and 82.26%when the tumor diameter was smaller than 4cm,with statistically significant differences(x2=5.615,P<0.05).TNM staging for gastric cancer ?-? midterm,VEGFR2 positive expression ratio were 71.43%,82.86%,95.65%and 100%respectively,statistically significant difference(x2=8.358,P<0.05);In the infiltration depth T1-T4,the positive VEGFR2 expression rates were 61.54%,84.21%,92.31%,and 96.88%,respectively,with statistically significant differences(x2=12.248,P<0.05).The percentage of VEGFR2 positive was 77.27%in the absence of vascular invasion and 96.61%in the presence of vascular invasion(x2=9.156,P<0.05).3.in carcinoma tissues,TNM staging ?-? midterm,EGFR positive proportion were 50.0%,68.6%,87.0%and 100%respectively,statistically significant difference(x2=12.016,p<0.05);In the infiltration depth T1-T4,the positive expression proportion of EGFR was 46.2%,68.4%,82.1%and 87.5%,respectively,the difference was statistically significant(x2=10.229,P<0.05).In the absence of lymph node metastasis,the positive EGFR expression rate was 67.9%,while in the presence of lymyh node metastasis,the positive EGFR exsression rate was 80.0%.The difference was statistically significant(x2=8.185,P<0.05).In the absence of vascular invasion,the EGFR positive expression accounted for 61.4%,while in the presence of vascular invasion,the EGFR positive expression accounted for 88.1%,with statistically significant difference(x2=10.108,P<0.05).4.The mean relapse-free survival of the VEGFR2-negative group and the VEGFR2-ositive group was 33.750 and 22.257 months,respectively,with statistically significant differences(P<0.05).The mean relapse-free survival of patients in the EGFR negative expression group and the EGFR positive group was 30.859 months and 23.960 months,with statistically significant differences(P<0.05).5.Spearman's rank correlation analysis showed a positive correlation between VEGFR2 and EGFR expression in gastric cancer tissues(correlation coefficient R=0.373,P<0.001).6.The TNM stage was worse when VEGFR2 and EGFR were both positive than when VEGFR2 and EGFR were both positive(r=0.381,P<0.001),and the mean relapse-free survival was shorter(mean relapse-free survival was 14.45 months with double positive).Single positive for 22.00 months and double negative for 33.63 months).conclusion:1.The expression intensity of VEGFR2 was higher in gastric cancer tissues than in adjacent tissues.The larger the tumor diameter,the higher TNM stage,the deeper the invasion and the higher the expression level of VEGFR2 in the presence of vascular invasion2.EGFR is highly expressed in gastric cancer tissues,and the expression intensity is higher than that in adjacent tissues.The deeper the infiltration is,the higher the TNM stage is,and the higher the expression level of EGFR is in the case of lymphatic metastasis and vascular invasion.3.The mean recurrence-free survival time of patients with positive expression of VEGFR2 and EGFR in gastric cancer tissues was significantly lower than that of patients with negative expression.4.There was a positive correlation between VEGFR2 expression and EGFR expression in gastric cancer tissues,providing a basis for screening targeted drugs in gastric cancer patients.