| Objective:To detect the variation of the expression of angiogenesis-related gene VEGFR2, EphrinB2between gastric carcinoma and normal gastric tissue, investigate the correlation of their expression and evaluate the relations of these genes with tumor differentiation, invasion, lymph node metastasis and TNM stage, to determine its role in malignant progression, and search a new target for clinic diagnosis and treatment of gastric carcinoma.Methods Carcinoma samples and surgical margin of stomach were collected from30patients who underwent surgery in our hospital from April2010to March2011. Real time quantitative RT-PCR and immunohistochemistry were used to detect the expression of VEGFR2and EphrinB2in these samples. The relations between these genes and the relationship between their expressions and the pathological data were analyzed.Results The expression of mRNA and protein of VEGFR2and EphrinB2in carcinoma were significantly higher than those in tissues of surgical margin of stomach (P<0.05).There was significantly correlation between the expression of VEGFR2and EphrinB2of mRNA and protein in carcinoma and TNM stage, depth of primary tumor invasion and lymph node metastasis (P<0.05), but no correlation with age, gender, site of tumor and differentitiation (P>0.05). In gastric carcinoma, there was positive correlation between VEGFR2and EphrinB2of mRNA and protein (P<0.05).Conclusion The VEGFR2and EphrinB2are overexpressed in gastric carcinoma on both mRNA and protein level, which shows that these genes play important roles in the process of tumor angiogenesis. Controlling tumor angiogenesis by targeting these genes may be a new approach for therapy of gastric carcinoma. Meanwhile, the expression of VEGFR2and EphrinB2correlates with depth of primary tumor invasion and TNM stage, which indicates that these genes play a crucial role in gastric cancer’s progression and invasion and they have good pathoclinical diagnostic value. |
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